Cross-linking of VLA/CD29 molecule has a co-mitogenic effect with anti-CD3 on CD4 cell activation in serum-free culture system.
Cross-linking of VLA/CD29 molecule has a co-mitogenic effect with anti-CD3 on CD4 cell activation in serum-free culture system.
复制标题
VLA/CD29分子的交联与抗CD3对无血清培养系统中CD4细胞的活化具有共同有丝分裂作用。
DOI：
10.1002/eji.1830210212
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发表时间：
1991
影响因子：
5.4
通讯作者：
Morimoto,C
中科院分区：
文献类型：
--
作者：
Yamada,A;Nojima,Y;Sugita,K;Dang,NH;Schlossman,SF;Morimoto,C
在这项研究中，我们证明了与VLA抗原家族的一个共同β1亚基（CD29）反应的抗体可以与CD3协同作用，但不能与CD2协同作用，在无血清培养系统中诱导CD4细胞增殖。激活程度与用抗CD3 +纤维连接蛋白培养CD4细胞时相似。通过CD3‐CD29途径诱导的增殖反应与白细胞介素（IL） 2自分泌途径相关，包括IL2的产生和IL2受体的表达。具体来说，VLA - 5，而不是VLA家族抗原的其他成员与CD3分子的交联诱导CD4细胞的强烈激活。然而，在无血清培养系统中，CD3与其他细胞表面抗原（如CD4）交联不会诱导CD4细胞活化。上述结果强烈提示VLA/CD29家族抗原可能通过CD3‐T细胞受体途径在调节CD4 T细胞活化中发挥重要作用。
In this study, we demonstrated that antibodies reactive with a common β1subunit (CD29) of the VLA family of antigens could synergize with CD3 but not CD2 in inducing CD4 cell proliferation in a serum‐free culture system. The extent of activation was similar to that obtained when CD4 cells were cultured with anti‐CD3 plus fibronectin. The proliferative response induced via the CD3‐CD29 pathway was associated with the interleukin (IL) 2 autocrine pathway, including IL2 production and IL2 receptor expression. Specifically, cross‐linking of VLA‐5, but not other members of the VLA family of antigens with the CD3 molecule induced strong CD4 cell activation. However, cross‐linking of CD3 with other cell surface antigens such as CD4 did not induce CD4 cell activation in this serum‐free culture system. The above results strongly suggest that the VLA/CD29 family of antigens may play an important role in regulating CD4 T cell activation via the CD3‐T cell receptor pathway.