Photoperiod pathway regulates expression of MAF5 and FLC that encode MADS-box transcription factors of the FLC family in Arabidopsis
Photoperiod pathway regulates expression of MAF5 and FLC that encode MADS-box transcription factors of the FLC family in Arabidopsis
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DOI：
10.5511/plantbiotechnology.10.0823b
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发表时间：
2010-01-01
影响因子：
1.6
通讯作者：
Mizoguchi, Tsuyoshi
中科院分区：
文献类型：
--
作者：
Fujiwara, Sumire;Nakagawa, Mayu;Mizoguchi, Tsuyoshi
日照长度对开花的控制是第一个被描述的光周期反应，也是许多植物中最具特征的反应之一。生物钟在光周期开花途径中起着关键作用。在拟南芥中，开花时间受光周期以及赤霉酸和春化/自主途径控制。FLC是一种MADS盒蛋白，是春化/自主途径中的一个关键花抑制因子。虽然花激活剂GIGANTEA，CONSTANS和FLOWERSHIP LOCUS T在光周期开花途径中的作用和调节已经得到很好的表征，但花抑制剂的作用和调节还不清楚。在这里，我们证明了MADS影响FLC家族成员之一的FLC基因5（MAF 5）在光/暗周期中表现出昼夜表达模式，并且光周期途径中的功能获得和丧失突变都会影响MAF 5和FLC的基因表达。这些结果突出了MAF 5和FLC在拟南芥光周期和春化/自主途径之间的串扰中可能的作用。
The control of flowering by day length was the first photoperiodic response to be described and is also one of the most characterized in many plants. The circadian clock plays a pivotal role in the photoperiodic flowering pathway. In Arabidopsis, flowering time is controlled by the photoperiod as well as by the gibberellic acid and vernalization/autonomous pathways. FLOWERING LOCUS C (FLC), a MADS box protein, has been shown to be a key floral repressor in the vernalization/autonomous pathways. Although the roles and regulation of floral activators GIGANTEA, CONSTANS, and FLOWERING LOCUS T in the photoperiodic flowering pathway have been well characterized, those of the floral repressors are not well understood. Here, we demonstrate that the MADS AFFECTING FLOWERING 5 (MAF5) gene, one of the FLC family members, shows a diurnal expression pattern in light/dark cycles and that both gain-and loss-of-function mutations in the photoperiod pathway affect the gene expression of MAF5 and FLC. These results highlight the possible roles of MAF5 and FLC in crosstalk between the photoperiod and vernalization/autonomous pathways in Arabidopsis.