Following the Fate of Dye-Containing Liposomes In Vitro
Following the Fate of Dye-Containing Liposomes In Vitro
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DOI：
10.3390/ijms21144847
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发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Skalko-Basnet, Natasa
中科院分区：
文献类型：
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作者：
Cauzzo, Jennifer;Nystad, Mona;Skalko-Basnet, Natasa
从基础研究到临床应用的翻译相当有限的成功已经被强调为纳米医学领域的一个主要问题。为了确定影响纳米系统作为药物载体和潜在纳米医学的适用性的因素，我们专注于通过基于荧光的测定，即流式细胞术和成像来跟踪它们的命运。这些方法通常用于跟踪纳米载体的内化和靶向;然而，所获得的结果的有效性严格取决于从荧光染料的命运可以推断出多少纳米系统的命运。为了评估影响标记纳米系统的物理化学和生物学稳定性的参数，我们利用明确定义的体外测定研究了两种脂质染料TopFluor（R）-PC和Cy 5-DSPE在常规脂质体中的通用性。我们的研究结果表明，染料可以影响系统的主要特征，如囊泡的大小和zeta电位。然而，纳米载体也可以影响染料性质。介质、温度、时间、荧光团定位和浓度以及它们之间的相互作用影响示踪实验的结果。因此，标记的纳米系统的深入表征应该是理解在优化纳米载体的筛选过程中验证结果的条件的基础。
The rather limited success of translation from basic research to clinical application has been highlighted as a major issue in the nanomedicine field. To identify the factors influencing the applicability of nanosystems as drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry and imaging. These methods are often used to follow the nanocarrier internalization and targeting; however, the validity of the obtained results strictly depends on how much the nanosystem's fate can be inferred from the fate of fluorescent dyes. To evaluate the parameters that affect the physicochemical and biological stability of the labeled nanosystems, we studied the versatility of two lipid dyes, TopFluor(R)-PC and Cy5-DSPE, in conventional liposomes utilizing well-defined in vitro assays. Our results suggest that the dye can affect the major characteristics of the system, such as vesicle size and zeta-potential. However, a nanocarrier can also affect the dye properties. Medium, temperature, time, fluorophore localization and its concentration, as well as their interplay, affect the outcome of tracing experiments. Therefore, an in-depth characterization of the labeled nanosystem should be fundamental to understand the conditions that validate the results within the screening process in optimization of nanocarrier.