Efficient Recovery of Glucose and Fructose via Enzymatic Saccharification of Rice Straw with Soft Carbohydrates
Efficient Recovery of Glucose and Fructose via Enzymatic Saccharification of Rice Straw with Soft Carbohydrates
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DOI：
10.1271/bbb.80840
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发表时间：
2009-05-01
影响因子：
1.6
通讯作者：
Tokuyasu, Ken
中科院分区：
文献类型：
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作者：
Park, Jeung-yil;Seyama, Tomoko;Tokuyasu, Ken
软碳水化合物，定义为通过从生物质中提取或简单的酶糖化容易回收的碳水化合物，在稻草中以游离葡萄糖、游离果糖、蔗糖、淀粉和β-1，3-1，4-葡聚糖的形式大量存在。在这项研究中，我们调查了它们的量在稻草（定义为秆和叶鞘），并开发了一种简单的方法，从他们的葡萄糖和果糖回收热预处理和随后的4小时酶糖化与酶的混合物的纤维素酶和淀粉葡萄糖苷酶。葡萄糖和果糖的回收率与软质碳水化合物的量具有良好的相关性。抽穗期和成熟期单位干重的葡萄糖和果糖产量最高的品种为cv。在cv.叶星因此，含有软质碳水化合物的稻草可以被视为经济可行的生产易于发酵的葡萄糖和果糖用于生物乙醇的新型原料。
Soft carbohydrates, defined as readily-recoverable carbohydrates via mere extraction from the biomass or brief enzymatic saccharification, were found in significant amounts in rice straw as forms of free glucose, free fructose, sucrose, starch, and beta-1,3-1,4-glucan. In this study, we investigated their amounts in rice straw (defined as culm and leaf sheath), and developed an easy method for glucose and fructose recovery from them with heat-pretreatment and subsequent 4-h enzymatic saccharification with an enzyme cocktail of cellulase and amyloglucosidase. The recovery of glucose and fructose exhibited good correlation with the amounts of soft carbohydrates. The maximum yields of glucose and fructose in the rice straw per dry weight at the heading stage and the mature stage were 43.5% in cv. Habataki and 34.1% in cv. Leafstar. Thus, rice straw with soft carbohydrates can be regarded as a novel feedstock for economically feasible production of readily-fermentable glucose and fructose for bioethanol.