Sonic hedgehog, secreted by amacrine cells, acts as a short-range signal to direct differentiation and lamination in the zebrafish retina
Sonic hedgehog, secreted by amacrine cells, acts as a short-range signal to direct differentiation and lamination in the zebrafish retina
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DOI：
10.1242/dev.01247
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发表时间：
2004-08-01
期刊：
影响因子：
4.6
通讯作者：
Neumann, CJ
中科院分区：
文献类型：
--
作者：
Shkumatava, A;Fischer, S;Neumann, CJ
斑马鱼视网膜中的神经发生经历了几个分化波。第一个神经源性波产生神经节细胞，依赖于刺猬(HH)信号活动。利用在sonic hedgehog(Shh)启动子控制下表达GFP的转基因斑马鱼胚胎，我们对视网膜内的分化波进行了成像，结果表明，除了节细胞层的分化波外，shh的表达还扩散到内核层。第二波产生表达shh的无长突细胞，虽然它与第一波在时间上重叠，但它不依赖于第一波，因为它发生在没有神经节细胞的情况下。我们还表明，内核层和外核层中的细胞类型的分化，以及视网膜的层叠，取决于shh。通过进行镶嵌分析，我们证明Shh在神经视网膜内将这些事件作为短程信号进行引导。
Neurogenesis in the zebrafish retina occurs in several waves of differentiation. The first neurogenic wave generates ganglion cells and depends on hedgehog (hh) signaling activity. Using transgenic zebrafish embryos that express GFP under the control of the sonic hedgehog (shh) promoter, we imaged the differentiation wave in the retina and show that, in addition to the wave in the ganglion cell layer, shh expression also spreads in the inner nuclear layer. This second wave generates amacrine cells expressing shh, and although it overlaps temporally with the first wave, it does not depend on it, as it occurs in the absence of ganglion cells. We also show that differentiation of cell types found in the inner and outer nuclear layers, as well as lamination of the retina, depends on shh. By performing mosaic analysis, we demonstrate that Shh directs these events as a short-range signal within the neural retina.