Association between the sinus microbiota with eosinophilic inflammation and prognosis in chronic rhinosinusitis with nasal polyps
Association between the sinus microbiota with eosinophilic inflammation and prognosis in chronic rhinosinusitis with nasal polyps
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DOI：
10.1038/s12276-020-0458-1
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发表时间：
2020-06-29
影响因子：
12.8
通讯作者：
Jang, Yong Ju
中科院分区：
文献类型：
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作者：
Kim, Ji Heui;Kim, Sung Hee;Jang, Yong Ju
鼻窦微生物组的生态失调影响慢性鼻窦炎伴鼻息肉（CRSwNP）的病理生理学。我们研究了CRSwNP中的窦微生物群是否与嗜酸性粒细胞炎症相关，特别是与先天性淋巴细胞（ILC），预后和血清细胞外囊泡（EV）相关。通过16 S核糖体RNA测序分析了31名CRSwNP患者和6名健康对照的中鼻道拭子和血清。采用流式细胞术和ELISA法分别检测鼻黏膜ILC 2和细胞因子的表达。根据嗜酸性粒细胞炎症和手术结果比较细菌的相对丰度（RA）。分析鼻窦细菌与ILC 2、细胞因子及血清EV的相关性。在属的水平上，各组鼻窦细菌的组成存在差异。嗜酸性粒细胞CRSwNPs组厌氧菌RA较对照组显著降低（P = 0.010），而毛梭菌RA较对照组显著升高（P = 0.038）。毛梭菌的RA与IL-5产生的ILC 2数量呈显著正相关（R = 0.340，P = 0.049），而厌氧菌的RA与IL-5产生的ILC 2数量呈显著负相关（R =-0.332，P = 0.055）。与最佳结果患者和对照组相比，次佳结果患者的棒状杆菌、厌氧球菌和Tepidimas的RA显著降低。部分鼻窦细菌与血清EV呈正相关。CRSwNP患者显示出基于与ILC 2和手术结果相关的嗜酸性炎症的不同微生物群组成。这些发现支持CRSwNPs中的微生物群和宿主免疫应答之间的关系。鼻窦疾病：与慢性疾病表现相关的鼻细菌生活在鼻窦腔中的微生物似乎会影响患有炎症性疾病的患者的免疫应答和临床结果，该炎症性疾病可导致鼻窦中的持续疼痛、压力和肿胀。来自韩国首尔牙山医疗中心的Ji Heui Kim及其同事收集了31名患有慢性鼻窦炎伴鼻息肉的患者和6名健康对照者的鼻拭子和血液。他们确定了几个特定的细菌群，它们的存在或不存在与某些白色血细胞群的丰度有关，这是炎症的标志。其中一些组与鼻部手术后的患者结局有关，但也观察到其他组。这些发现可能有助于为这种鼻窦疾病患者带来新的治疗策略或诊断生物标志物。
Dysbiosis of the sinus microbiome affects the pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNPs). We investigated whether the sinus microbiota in CRSwNPs is associated with eosinophilic inflammation, especially in relation to innate lymphoid cells (ILCs), prognosis, and serum extracellular vesicles (EVs). Middle meatal swabs and serum from 31 CRSwNPs patients and six healthy controls were analyzed by 16S ribosomal RNA sequencing. ILC2s and cytokines from sinonasal tissues were measured by flow cytometry and ELISA, respectively. The relative abundances (RAs) of bacteria were compared based on eosinophilic inflammation and surgical outcome. The correlations between sinus bacteria and ILC2s, cytokines, and serum EVs were analyzed. The compositions of sinus bacteria were different between groups at the genus level. In eosinophilic CRSwNPs patients, the RA ofAnaerococcuswas significantly decreased (P = 0.010), whereas that ofLachnoclostridiumwas significantly increased (P = 0.038) compared with that in controls. The RA ofLachnoclostridiumshowed a significant positive correlation with interleukin (IL)-5-producing ILC2 populations (R = 0.340,P = 0.049), whereas the RA ofAnaerococcusshowed a negative correlation with IL-5-producing ILC2 populations (R = -0.332,P = 0.055). The RAs ofCorynebacterium,Anaerococcus, andTepidimonaswere significantly decreased in patients with suboptimal outcomes compared with those in patients with optimal outcomes and control subjects. Some sinus bacteria and serum EVs showed positive correlations. CRSwNPs patients showed distinct microbiota compositions based on eosinophilic inflammation in relation to ILC2s and surgical outcome. These findings support a relationship between the microbiota and the host immune response in CRSwNPs.Sinus disease: nasal bacteria linked to manifestations of chronic disease Microbes living in the sinonasal cavity seem to affect the immune responses and clinical outcomes of patients with an inflammatory disease that can cause lasting pain, pressure, and swelling in the sinuses. Ji Heui Kim from the Asan Medical Center in Seoul, South Korea, and colleagues collected nasal swabs and blood from 31 people suffering from chronic rhinosinusitis with nasal polyps and from six healthy control individuals. They identified several specific bacterial groups whose presence or absence was associated with the abundance of certain white blood cell populations, a sign of inflammation. Some of these groups were linked to patient outcomes after nasal surgery but additional groups were also observed. The findings could help lead to new treatment strategies or diagnostic biomarkers for patients with this sinus disorder.