Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry
Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry
复制标题
石墨氮化碳改性中空纤维固相微萃取气相色谱-质谱联用萃取测定尿液和血清中的尿酸
DOI：
10.1016/j.jchromb.2015.09.025
复制
发表时间：
2015-11-01
影响因子：
3
通讯作者：
Shi, Yan-ping
中科院分区：
文献类型：
--
作者：
Sun, Ying-pei;Chen, Juan;Shi, Yan-ping
尿液或血清中尿酸(UA)升高可影响肾功能和血压，而血压是痛风、心血管和肾脏疾病、高血压等疾病的指标。本工作制备了一种基于氮化石墨(g-CNS)和中空纤维(HF)的新型混合基质膜(MMM)，并将其与固相微萃取(SPME)相结合，用于尿液和血清中UA的测定，然后用气相色谱-质谱仪(GC/MS)进行分析。将多孔g-CNS分散在氨水中，然后通过毛细管力和声学作用将剥离的g-CNS纳米片固定在HF的孔隙中。制备的g-CNS修饰HF(g-CNS-HF)直接浸泡在生物流体中，以SPME模式提取尿酸，无溶剂模式便于进一步的衍生化和分析。为了获得最高的提取效率，对g-CNS-HF的固定化时间、超声功率和提取时间、衍生化和解吸时间等主要提取和衍生化参数进行了优化。在最佳提取条件下，尿液和血清中尿酸的平均回收率分别为80.7%~121.6%和84.7%~101.1%，尿液和血清中尿酸在0.10~200mgmL(-1)范围内呈良好的线性关系，相关系数均大于0.9990。结果表明，所建立的g-CNS-HF-SPME是一种简单、快速、经济、无溶剂的分析生物体液中尿酸的方法。(C)2015爱思唯尔B.V.保留所有权利。
An elevated uric acid (UA) in urine or serum can affect renal function and blood pressure, which is an indicator of gout, cardiovascular and renal diseases, hypertension, etc. In this work, a new type of mixed matrix membrane (MMM), based on graphitic carbon nitrides (g-CNs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine UA in urine and serum followed by gas chromatography-mass spectrometry (GC/MS). The porous g-CNs were dispersed in ammonia, and then the exfoliated g-CNs nanosheets were held in the pores of HF by capillary forces and sonification. The prepared g-CNs modified HF (g-CNs-HF) was immersed in biofluid directly to extract UA with SPME mode and the solvent-free mode is convenient for further derivatization and analysis. To achieve the highest extraction efficiency (EF), main extraction and derivatization parameters, such as g-CNs-HF immobilizing time, sonification power and time of extraction, derivatization and desorption time, were optimized. Under the optimum extraction conditions, a favorable linearity of UA was obtained in the range 0.1-200 mu g mL(-1) with correlation coefficients higher than 0.9990, and the average recoveries at three spiked levels of UA in urine and serum ranged from 80.7% to 121.6%, from 84.7% to 101.1%, respectively. The obtained results demonstrated the developed g-CNs-HF-SPME is a simple, rapid, cost-effective, solvent-free method for the analysis of UA in biofluid. (C) 2015 Elsevier B.V. All rights reserved.