SKIP regulates environmental fitness and floral transition by forming two distinct complexes in Arabidopsis
SKIP regulates environmental fitness and floral transition by forming two distinct complexes in Arabidopsis
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SKIP 通过在拟南芥中形成两种不同的复合体来调节环境适应性和花的转变
DOI：
10.1111/nph.15990
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发表时间：
2019-10-01
期刊：
影响因子：
9.4
通讯作者：
Ma, Ligeng
中科院分区：
文献类型：
--
作者：
Li, Yan;Yang, Jing;Ma, Ligeng
SKI相互作用蛋白(SKIP)是基因表达的双功能调节因子，作为剪接体的一部分，并通过与早花7(ELF7)相互作用而作为转录激活因子发挥作用。MOS4相关复合体3A(MAC3A)和MAC3B与SKIP在物理和遗传上相互作用，介导拟南芥基因组中约50%的表达基因的选择性剪接，并需要一组与SKIP相似的基因的剪接。Skip与剪接因子和聚合酶相关因子1复合体(Paf1c)在物理和遗传上相互作用。然而，这些剪接因子并不与Paf1c组分在物理或遗传上相互作用。跳跃-剪接体复合体通过控制时钟和胁迫耐受相关基因编码的前-mRNAs的选择性剪接来调节生物钟功能和非生物胁迫反应。Skip-Paf1c复合体通过激活开花基因C(FLC)转录来调节花的转变。我们的数据表明，SKIP通过掺入两个不同的复合体来调节花朵过渡和环境适应性，这两个复合体分别在转录和转录后调节基因表达。在未来的研究中，人们将很有兴趣地发现，是否需要通过控制SKIP掺入剪接体或植物中的Paf1c来整合环境适应性和生长。
Ski-interacting protein (SKIP) is a bifunctional regulator of gene expression that works as a splicing factor as part of the spliceosome and as a transcriptional activator by interacting with EARLY FLOWERING 7 (ELF7). MOS4-Associated Complex 3A (MAC3A) and MAC3B interact physically and genetically with SKIP, mediate the alternative splicing of c. 50% of the expressed genes in the Arabidopsis genome, and are required for the splicing of a similar set of genes to that of SKIP. SKIP interacts physically and genetically with splicing factors and Polymerase-Associated Factor 1 complex (Paf1c) components. However, these splicing factors do not interact either physically or genetically with Paf1c components. The SKIP-spliceosome complex mediates circadian clock function and abiotic stress responses by controlling the alternative splicing of pre-mRNAs encoded by clock- and stress tolerance-related genes. The SKIP-Paf1c complex regulates the floral transition by activating FLOWERING LOCUS C (FLC) transcription. Our data reveal that SKIP regulates floral transition and environmental fitness via its incorporation into two distinct complexes that regulate gene expression transcriptionally and post-transcriptionally, respectively. It will be interesting to discover in future studies whether SKIP is required for integration of environmental fitness and growth by control of the incorporation of SKIP into spliceosome or Paf1c in plants.