Identification and evaluation of a dominant alga from municipal wastewater in removal of nutrients.
Identification and evaluation of a dominant alga from municipal wastewater in removal of nutrients.
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DOI：
10.2166/wst.2016.437
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Yixuan Yang;Fei Tang;Xiaoling Su;Hua Yin;Fei Ge
中科院分区：
文献类型：
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作者：
Yixuan Yang;Fei Tang;Xiaoling Su;Hua Yin;Fei Ge
为了获得更好的去除营养盐的藻类为基础的技术，从真实的城市污水中确定了一个占主导地位的藻类，并评估其去除低浓度的氮（NH+4或NO-3）和磷（PO 3 -4）的能力。结果表明，短鞘藻（Oedogoniumbrevicingulatum）是一种丝状的绿色真菌，经聚合酶链反应-变性梯度凝胶电泳鉴定，短鞘藻为某城市污水处理厂二级出水中的优势菌。对低浓度的NH+4、NO-3（≤5 mg N L-1）和PO 3 -4（≤0.5 mg P L-1）的去除率为100%。NH 4+和NO3-的最大去除速率（Vmax）和半饱和常数（Km）分别为10.03 ± 0.95 mg g-1d-1和0.19 ± 0.03 mg L-1和8.43 ± 0.21 mg g-1d-1和0.27 ± 0.11 mg L-1，表明该系统对NH 4+的吸收能力高于NO3-.以NH+4为氮源时，该酶对PO 3 -4的亲和力（Vmax：1.42 ± 0.02 mg g-1 d-1; Km：0.02 ± 0.00 mg L-1）高于以NO-3为氮源时的亲和力（Vmax：1.24 ± 0.15 mg g-1 d-1; Km：0.06 ± 0.03 mg L-1）。氮磷比在5：1 ~ 20：1范围内，氮磷去除效果稳定。这些结果表明，城市污水中的优势藻类具有应用于去除营养盐的潜力。
To access better removal of nutrients with algae-based techniques, a dominant alga from real municipal wastewater was identified and its capacity in removing low concentrations of nitrogen (NH+4 or NO-3) and phosphorus (PO3-4) was evaluated. Results showed that Oedogonium brevicingulatum, a filamentous green alga, was confirmed as the dominant alga in the secondary effluent of a municipal wastewater treatment plant by polymerase chain reaction-denaturing gradient gel electrophoresis. Low concentrations of NH+4 or NO-3 (≤5 mg N L-1) and PO3-4 (≤0.5 mg P L-1) were 100% removed by the algae in a 7-d test. The maximum nutrient removal rate (Vmax) and the half-saturation constant (Km) for NH+4 (10.03 ± 0.95 mg g-1d-1 and 0.19 ± 0.03 mg L-1) and NO-3 (8.43 ± 0.21 mg g-1 d-1 and 0.27 ± 0.11 mg L-1) indicated the uptake capability for NH+4 is higher than that for NO-3. Meanwhile, it showed higher affinity for PO3-4 (Vmax: 1.42 ± 0.02 mg g-1 d-1; Km: 0.02 ± 0.00 mg L-1) with NH+4 as nitrogen source than that (Vmax: 1.24 ± 0.15 mg g-1 d-1; Km: 0.06 ± 0.03 mg L-1) with NO-3 as nitrogen source. Moreover, nutrient removal efficiencies were observed steady when nitrogen/phosphorus ratio ranged from 5:1 to 20:1. These results suggest that the dominant algae from municipal wastewater have potentials to be applied in nutrient removal.