Antibiofilm effect of ultrasound combined with microbubbles against Staphylococcus epidermidis biofilm
Antibiofilm effect of ultrasound combined with microbubbles against Staphylococcus epidermidis biofilm
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超声联合微泡对表皮葡萄球菌生物膜的抑菌作用
DOI：
10.1016/j.ijmm.2017.06.001
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发表时间：
2017-09-01
影响因子：
4.1
通讯作者：
Yu, Jialin
中科院分区：
文献类型：
--
作者：
Dong, Ying;Xu, Yaling;Yu, Jialin
由于生物膜对抗生素和宿主免疫细胞具有抗性，因此很难根除。超声微泡已成为一种新的治疗方式，其潜在机制尚不清楚。在这项研究中，我们将 OptiCell 腔室中建立的 24 小时表皮葡萄球菌生物膜暴露于超声波与微泡的结合下，并研究了处理后万古霉素和中性粒细胞对表皮葡萄球菌生物膜的活性。从细菌渗透性和抗菌性方面探讨了超声波微泡的抗菌膜机制。 生物膜相关基因表达。超声（1 MHz、0.5 W/cm(2)、50%占空比）联合浓度为1%和4%（v/v）的微泡处理5 min后，细菌对胞外荧光染料的通透性增强，icaA表达下调，agrB和RNAIII表达上调。后处理 与单独接触万古霉素的生物膜相比，生物膜对万古霉素更敏感，其生物量减少（P < 0.05）。中性粒细胞响应生物膜的吞噬作用、氧化爆发活性以及趋化性也显着增加。超声联合微泡的生物效应一般比单独超声更显着，且依赖于微泡 浓度。这项研究表明，超声波与微泡相结合可以通过机械和生化机制增强抗生素和中性粒细胞对抗生物膜的活性，并且可以提供除化学和生物方法之外的有效且非侵入性的抗生物膜替代方案。
Biofilms are difficult to eradicate due to their resistance to antibiotics and host immune cells. Ultrasound microbubbles have emerged as a new treatment modality with the underlying mechanisms largely unknown. In this study, we exposed 24-h-old Staphylococcus epidermidis biofilms established in OptiCell" chambers to ultrasound in combination with microbubbles, and investigated the activities of vancomycin and neutrophils against S. epidermidis biofllms after treatment. The antibiofilm mechanims of ultrasound microbubbles were explored in terms of bacterial permeability and biofilm-associated gene expression. After treatment of ultrasound (1 MHz, 0.5 W/cm(2), 50% duty cycle) combined with microbubbles in the concentration of 1% and 4% (v/v) for 5 min, bacterial permeability to extracellular fluorescent dyes was enhanced and the expression of icaA was down regulated while that of agrB and RNAIII up-regulated. Post-treatment biofllms were more sensitive to vancomycin by demonstrating reduced biomass than those exposed to vancomycin alone (P < 0.05). The phagocytosis, oxidative burst activity as well as chemotaxis of neutrophils in response to biofllms were also significantly increased. The bioeffect of ultrasound combined with microbubbles was generally more significant than that of ultrasound alone, and dependent on microbubble concentration. This study demonstrated that ultrasound combined with microbubbles could enhance the activities of antibiotics and neutrophils against biofllms possibly via mechanical and biochemical mechanisms, and may provide an efficient and non-invasive antibiofilm alternative apart from chemical and biological approaches.