Kobuvirus shedding dynamics in a swine production system and their association with diarrhea
Kobuvirus shedding dynamics in a swine production system and their association with diarrhea
复制标题
DOI：
10.1016/j.vetmic.2019.07.023
复制
发表时间：
2019-08-01
影响因子：
3.3
通讯作者：
Brassard, Julie
中科院分区：
文献类型：
--
作者：
Nantel-Fortier, Nicolas;Lachapelle, Virginie;Brassard, Julie
猪柯布病毒在猪中广泛分布，但这些病毒的临床意义尚不清楚，因为它们与腹泻猪和健康猪都有关联。此外，关于加拿大猪群中柯布病毒流行的数据也很少。本研究共对181头腹泻健康的仔猪进行了四次监测和抽样，以代表生产的不同阶段。在饲养场(出生至断奶阶段)、苗圃场(断奶后阶段)和育肥场(育肥阶段开始和结束)对仔猪进行取样。在每个生命阶段都采集了粪便和环境样本。病毒提取后，用RT-PCR检测柯布病毒，并对阳性标本进行测序。在哺乳后期(6-21日龄)，腹泻仔猪比健康个体排出更多的柯布病毒。仔猪在断奶后阶段(苗圃)排出的柯布病毒比其他任何生命阶段都要多。这一点在个别样本和环境样本中都得到了证明。超过97%的抽样仔猪一生中至少排出一次柯布病毒。在哺乳阶段排出一种柯布病毒株或混合株的所有仔猪，在以后的生命阶段似乎没有排出另一种猪柯布病毒株。总体而言，我们的发现揭示了柯布病毒在哺乳阶段的脱落动力学及其在新生儿腹泻中的潜在作用，这似乎是柯布病毒进入生猪生产系统的切入点。
Porcine kobuviruses are widely distributed in swine, but the clinical significance of these viruses remains unclear, since they have been associated with both diarrheic and healthy pigs. In addition, there is a paucity of data on Kobuvirus prevalence in Canadian pig herds. In this study, a total of 181 diarrheic and healthy piglets were monitored and sampled on four occasions, intended to represent the different stages of production. The piglets were sampled at the nursing farms (birth to weaning stage), at the nursery farms (post-weaning stage), and at finishing farms (at the beginning and the end of the fattening stage). Fecal and environmental samples were collected during each life stage. Following viral extraction, Kobuvirus detection by RT-PCR was conducted, and positive samples were sequenced. During the late-nursing stage (6-21 days old), piglets with diarrhea shed more Kobuvirus than healthy individuals. Piglets shed more Kobuvirus during the post-weaning stage (nursery farms) than during any of the other life stages. This was evidenced in individual samples as well as in environmental samples. Over 97% of the sampled piglets shed Kobuvirus at least once in their lifetime. All piglets shedding a Kobuvirus strain or mix of strains at the nursing stage did not appear to shed another porcine kobuvirus strain at a later life stage. Overall, our findings throw light on Kobuvirus shedding dynamics and their potential role in neonatal diarrhea at the nursing stage, which appears to be the point of entry for kobuviruses into swine production systems.