A METHOD FOR THE ESTIMATION OF POLYETHYLENE-GLYCOL IN PLASMA-PROTEIN FRACTIONS
A METHOD FOR THE ESTIMATION OF POLYETHYLENE-GLYCOL IN PLASMA-PROTEIN FRACTIONS
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DOI：
10.1016/0003-2697(80)90492-3
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
SNAPE, TJ
中科院分区：
文献类型：
--
作者：
SIMS, GEC;SNAPE, TJ
当含有PEG的样品中存在蛋白质时，PEG[聚乙二醇]估计的不稳定结果可能出于以下两个原因之一。在尝试用TCA[三氯乙酸]沉淀后，任何留在溶液中的蛋白质，无论是否存在PEG，都可能增加最终反应的光密度。聚乙二醇倾向于与蛋白质沉淀物共沉淀或吸附在蛋白质沉淀物上。如果将原始样品[人血清]稀释至含有不超过3.0 mg蛋白质/ml，则可以定量回收PEG。
Erratic results in PEG [polyethylene glycol] estimation may be obtained for 1 of 2 reasons when protein is present in the PEG-containing sample. Any protein which remains in solution after attempted precipitation with TCA [trichloroacetic acid] may increase the optical density in the final reaction whether or not PEG is present. PEG tends to be coprecipitated with, or adsorbed on to, protein precipitates. Quantitative recovery of PEG is possible if the original sample [human serum] is diluted to contain not more than 3.0 mg protein/ml.