von Willebrand factor is present on the surface of platelets stimulated in plasma by ADP.
von Willebrand factor is present on the surface of platelets stimulated in plasma by ADP.
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血管性血友病因子存在于 ADP 刺激的血浆血小板表面。
DOI：
10.1182/blood.v70.5.1362.bloodjournal7051362
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发表时间：
1987
期刊：
影响因子：
20.3
通讯作者：
P. Powers
中科院分区：
文献类型：
--
作者：
B. Adelman;P. Carlson;P. Powers
血管性血友病因子（vWf）可与活化血小板上的糖蛋白（GP）IIb/IIIa结合。然而，这种相互作用的意义尚不清楚，因为尚不可能检测到vWf与血浆中刺激的血小板上的GPIIb/IIIa的结合。我们已经开发了一种间接的流式细胞术检测，使用荧光素标记的抗体来检测血小板上的vWf和纤维蛋白原。用这种方法，我们发现vWf的血小板表面上的ADP刺激的血浆。结合vWf的血小板数量与ADP浓度和孵育时间成比例增加。在1 μ mol/L钙离子载体A23187或10 μ mol/L ADP激活的无蛋白缓冲液中洗涤的血小板也结合vWf，这表明我们检测到α颗粒衍生的vWf的表面结合。抗GPIb上vWf结合位点（6D 1）和GPIIb/IIIa上vWf和纤维蛋白原结合位点（分别为LJP 5和LJ-CP 8）的单克隆抗体用于表征vWf与刺激血小板结合的机制。瑞斯托菌素诱导的vWf结合被6D 1抑制，ADP诱导的纤维蛋白原结合被LJ-CP 8抑制。这些抗体均不抑制ADP诱导的vWf结合。阿司匹林和前列腺素E1也抑制ADP诱导的富血小板血浆中vWf的结合。在血浆中的血小板活化期间，源自α-颗粒的vWf变得与血小板表面结合，可能已经与结合位点相关联地被转移。
von Willebrand factor (vWf) can bind to glycoprotein (GP) IIb/IIIa on activated platelets. The significance of this interaction is unclear, however, because it has not been possible to detect vWf binding to GPIIb/IIIa on platelets stimulated in plasma. We have developed an indirect, flow cytometry assay that uses fluorescein-labeled antibodies to detect vWf and fibrinogen on platelets. Using this assay, we found vWf on the surface of platelets stimulated in plasma by ADP. The number of platelets that bound vWf increased in proportion to ADP concentration and incubation time. Washed platelets in a protein-free buffer activated by 1 mumol/L calcium ionophore A23187 or 10 mumol/L ADP also bound vWf, suggesting that we were detecting surface binding of alpha-granule-derived vWf. Monoclonal antibodies against the vWf binding site on GPIb (6D1) and the vWf and fibrinogen binding sites on GPIIb/IIIa (LJP5 and LJ-CP8, respectively) were used to characterize the mechanism of vWf binding to stimulated platelets. Ristocetin-induced binding of vWf was inhibited by 6D1, and ADP-induced binding of fibrinogen was inhibited by LJ-CP8. None of these antibodies inhibited ADP-induced vWf binding. Aspirin and prostaglandin E1 also inhibited ADP-induced binding of vWf in platelet-rich plasma. During platelet activation in plasma, vWf derived from alpha-granules becomes bound to the platelet surface possibly being transferred already associated with a binding site.
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血小板因子 VIII/冯维勒布兰德因子的多聚体结构：较大多聚体的存在及其与凝血酶刺激的血小板的重新结合。
影响因子：
20.3
作者：
Fernandez,MF;Ginsberg,MH;Ruggeri,ZM;Batlle,FJ;Zimmerman,TS
通讯作者：
Zimmerman,TS
冯维勒布兰德因子和纤维蛋白原与人血小板结合的 ADP 依赖性共同受体机制。
DOI：
10.1073/pnas.81.15.4935
发表时间：
1984
影响因子：
11.1
作者：
Timmons,S;Kloczewiak,M;Hawiger,J
通讯作者：
Hawiger,J
ADP 分泌和血栓素合成在因子 VIII 与血小板结合中的作用。
影响因子：
20.3
作者：
DiMinno,G;Shapiro,SS;Catalano,PM;DeMarco,L;Murphy,S
通讯作者：
Murphy,S
单克隆抗体证明了血管性血友病因子和纤维蛋白原与血小板膜糖蛋白 IIb/IIIa 复合物结合的独立调节。
DOI：
10.1172/jci112193
发表时间：
1985
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Lombardo,VT;Hodson,E;Roberts,JR;Kunicki,TJ;Zimmerman,TS;Ruggeri,ZM
通讯作者：
Ruggeri,ZM
影响因子：
15.9
作者：
DEMARCO, L;GIROLAMI, A;RUGGERI, ZM
通讯作者：
RUGGERI, ZM