Molecular data supports monophyly of Triatoma dispar complex within genus Triatoma.
Molecular data supports monophyly of Triatoma dispar complex within genus Triatoma.
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分子数据支持锥蝽属内锥蝽复合体的单系性。
DOI：
10.1016/j.meegid.2020.104429
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Brenière,SimoneFrédérique
中科院分区：
文献类型：
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作者：
Santillán-Guayasamín,Soledad;Barnabé,Christian;Magallón-Gastelum,Ezequiel;Waleckx,Etienne;Yumiseva,CésarA;Grijalva,MarioJ;Villacís,AnitaG;Brenière,SimoneFrédérique
锥蝽属（Triatoma）是锥蝽族（Triatomini）的一个重要类群，根据形态学和地理学特征，将其分为北美锥蝽族（Triatomini）、南美锥蝽族（Triatomini）和南美锥蝽族（Triatomini）三大类群。dispar复合体T. dispar complex分布于厄瓜多尔。这种复合物几乎没有通过分子方法进行研究，这种复合物的分类位置也没有得到证实。本研究探讨了锥口鲶属内的系统发育关系，包括5个来自北美和中美洲的种类，6个来自南美洲的种类，以及3个属于锥口鲶属的种类。dispar复合体对74例标本进行了线粒体基因（Cyt B、COII、16 S-rRNA、12 S-rRNA）和核基因（18 S-rRNA、ITS 2）的部分序列测定。通过最大似然法（ML）、最大简约法（MP）和贝叶斯方法构建了串联序列和单个序列的系统发育树。使用串联序列构建的树显示了三个主要分支（簇），这些分支得到了显著自举值的高度支持; dispar复合体作为一个单系群出现，与北美洲和中美洲起源的物种和南美洲起源的物种分开。相反，对于每个基因树，三个主要的聚类并不总是得到显著的支持，主要是因为当考虑单个基因时，遗传信息急剧减少。因此，串联的基因给出了相关的结果，并强烈建议进一步深入研究的几个物种的关系和复杂的锥蝽，仍然没有解决。此外，我们目前的分子数据充分揭示了Triatoma属至少分为三个主要的遗传群。
The genus Triatoma contains numerous species, principal or secondary vectors of Chagas disease, which have been included in the three main lineages of Triatomini tribe based on morphological and biogeographical characteristics: North American, South American, and T. dispar complex. The three members of the T. dispar complex are distributed in Ecuador. This complex has been scarcely studied through molecular approaches, and the taxonomic position of this complex is not confirmed. In this study, we explored the phylogenetic relationships within the genus Triatoma, including five species from North and Central America, six from South America, and the three species belonging to the T. dispar complex. Partial sequences of four mitochondrial genes (Cyt b, COII, 16S-rRNA, 12S-rRNA) and two nuclear genes (18S-rRNA, ITS2) were obtained from 74 specimens. Phylogenetic trees were built with concatenated and single sequences through maximum likelihood (ML), maximum parsimony (MP), and Bayesian methods. The trees built using concatenated sequences showed three main branches (clusters) highly supported by significant bootstrap values; the T. dispar complex appeared as a monophyletic group separate from species of North and Central American origin and South American origin. On the contrary, for each gene tree, the three main clusters were not always significantly supported, mostly because genetic information is dramatically reduced when a single gene is considered. Consequently, concatenation of genes gives relevant results and is highly recommended for further in-depth examination of the relationships of several species and complexes of triatomines that remain unresolved. Moreover, our current molecular data fully revealed the division of genus Triatoma into at least three main genetic groups.