EVIDENCE FOR MACROPHAGE-MEDIATED PROTECTION AGAINST LETHAL CANDIDA-ALBICANS INFECTION
EVIDENCE FOR MACROPHAGE-MEDIATED PROTECTION AGAINST LETHAL CANDIDA-ALBICANS INFECTION
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DOI：
10.1128/iai.51.2.668-674.1986
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发表时间：
1986-02-01
影响因子：
3.1
通讯作者：
CASSONE, A
中科院分区：
文献类型：
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作者：
BISTONI, F;VECCHIARELLI, A;CASSONE, A
用不能酵母-菌丝体转化的白色念珠菌菌株(PCA-2)全身感染小鼠，可以保护小鼠免受随后静脉注射母体致病菌株CA-6的攻击。保护是非特异性的，因为它也在感染金黄色葡萄球菌的小鼠攻击时检测到。此外，PCA-2生物必须是活的，当它们在微生物挑战前7至14天以106个细胞的剂量静脉注射时，其效果最为明显。因此，所有用PCA-2预处理并在14天后用活的CA-6细胞攻击的小鼠都存活了60天的观察期，而所有没有接受PCA-2处理的对照组小鼠在3天内死亡。在试图将体内观察到的免疫刺激效应与体外功能可能的改变联系起来时，发现服用PCA-2会增加外周血中多形核细胞的数量，并在体外激活具有高杀菌活性的细胞。此外，将PCA-2感染的小鼠的塑料贴壁细胞过继转移到组织相容的受体中，可提供相当大的保护作用，抵御随后的CA-6挑战。
Systemic infection of mice with a Candida albicans strain (PCA-2) incapable of yeast-mycelial conversion conferred protection against a subsequent intravenous challenge with the pathogenic strain of the parent organism, strain CA-6. Protection was nonspecific since it was also detected upon challenge of mice with Staphylococcus aureus. Moreover, the PCA-2 organisms had to be viable, their effects being most evident when they were given intravenously at a dose of 106 cells 7 to 14 days prior to microbial challenge. Thus, all mice pretreated with PCA-2 and challenged 14 days later with viable CA-6 cells lived through a 60-day observation period, whereas all control mice not treated with PCA-2 died within 3 days. In an attempt to correlate the immunostimulatory effects observed in vivo with possible modifications in in vitro functions, it was found that administration of PCA-2 was accompanied by an increase in the number of peripheral blood polymorphonuclear cells and by the activation in the spleen of cells with highly candidacidal activity in vitro. Moreover, the adoptive transfer of plastic-adherent cells from PCA-2-infected mice into histocompatible recipients conferred considerable protection against subsequent CA-6 challenge.