Assembly of core helices and rapid tertiary folding of a small bacterial group I ribozyme
Assembly of core helices and rapid tertiary folding of a small bacterial group I ribozyme
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DOI：
10.1073/pnas.0337743100
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发表时间：
2003-02-18
影响因子：
11.1
通讯作者：
Woodson, SA
中科院分区：
文献类型：
--
作者：
Rangan, P;Masquida, B;Woodson, SA
紧凑但非天然的中间体涉及到几个大rna的分层折叠，但关于它们的结构的信息很少。在本文中，核糖核酸酶和羟基自由基切割保护实验表明，核心螺旋的碱基配对稳定了偶氮菌前trna （ile）的一小群I核酶的致密状态。核酶核心的碱基配对需要的Mg2+比稳定的三级相互作用少10倍，这表明催化核心中螺旋的组装代表了在形成天然三级结构之前的一个独特阶段。三级折叠发生在
Compact but non-native intermediates have been implicated in the hierarchical folding of several large RNAs, but there is little information on their structure. In this article, ribonuclease and hydroxyl radical cleavage protection assays showed that base pairing of core helices stabilize a compact state of a small group I ribozyme from Azoarcus pre-tRNA(ile). Base pairing of the ribozyme core requires 10-fold less Mg2+ than stable tertiary interactions, indicating that assembly of helices in the catalytic core represents a distinct phase that precedes the formation of native tertiary structure. Tertiary folding occurs in