Effects of UV-B radiation and different light repair conditions on the early development of the tetraspores of Chondrus ocellatus Holm
Effects of UV-B radiation and different light repair conditions on the early development of the tetraspores of Chondrus ocellatus Holm
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DOI：
10.1007/s13131-011-0124-5
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发表时间：
2011-07
影响因子：
1.4
通讯作者：
Qing Ju;Xue-Xi Tang;Xiaowei Zhao;Xiuquan Ren;Yunfei Li
中科院分区：
文献类型：
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作者：
Qing Ju;Xue-Xi Tang;Xiaowei Zhao;Xiuquan Ren;Yunfei Li
在实验室条件下，研究了280-315 nm的UV-B辐射和暗或暗修复条件对青岛沿海角叉菜（Chondrus ocellatusHolm）早期发育的影响。在不同剂量（0、36、72、108、144和180 J/（m2·d））的UV-B辐射下，将四分孢子分别置于正常培养条件（PAR）和黑暗条件下修复2 h。在培养过程中，每隔4d记录一次四孢子直径，直到第50天所有处理均形成垂直分枝。第50天测定叶绿素a、藻红蛋白、叶绿素a和单胺氧化酶活性（Palythine和asterina-330）。结果表明，低剂量的UV-B辐射能显著加快C.随着UV-B辐射强度的不断增加，其生长速率逐渐降低。藻红蛋白和叶绿素a浓度的变化趋势在UVB照射后均显著降低（P< 0.05）。在UV-B胁迫下，CPDs被诱导产生，且暗修复组CPDs浓度显著降低。利用LC/MS分析了C. ocellatus结果表明，UV-B辐射诱导后产生了两种MAAs（palythine和asterina-330），且低剂量的UV-B辐射能显著诱导MAAs的产生。从四分孢子的生长发育状况来看，UV-B辐射对暗修复组的负面影响比PAR严重。
The effects of exposure to UV-B radiation, 280–315 nm, and dark or dark repair conditions on the early development ofChondrus ocellatusHolm collected from Qingdao coast were studied under the laboratory condition. After being exposed to different doses [0, 36, 72, 108, 144 and 180 J/(m2·d)] of UV-B radiation, one treatment of tetraspores were put back to normal culture condition (PAR), the other treatment were put into dark condition to repair for 2 h. During the cultivation, the diameter of the tetraspores were recorded every 4 d till the 50th day when vertical branches formed in all treatments. Then at the 50th day, CPDs, phycoerythrin, chlorophyll a and MAAs (Palythine and asterina-330) were measured. The results showed that low doses UV-B radiation could significantly accelerate the growth rate of the tetraspores ofC. ocellatusand the growth rate reduced gradually with UV-B radiation keep on increasing. The variation trend of both the phycoerythrin and chlorophyll a concentration reduced significantly (P< 0.05) once given the UVB radiation. Under UV-B stress, the CPDs were induced, and the concentrations of CPDs were significant low in dark repair groups. The data of MAAs analyzed by LC/MS in the tetraspores ofC. ocellatussuggested that there were two MAAs formed (palythine and asterina-330) after UV-B radiation induced, and low dose of UV-B irradiation could significantly induced the concentration of MAAs. From the growth and development state of the tetraspores, negative effects of UV-B radiation on the dark repair groups were more serious than PAR.