Profiling Planktonic Biomass Using Element-Specific, Multicomponent Nuclear Magnetic Resonance Spectroscopy
Profiling Planktonic Biomass Using Element-Specific, Multicomponent Nuclear Magnetic Resonance Spectroscopy
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DOI：
10.1021/acs.est.5b00837
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发表时间：
2015-06-02
影响因子：
11.4
通讯作者：
Kikuchi, Jun
中科院分区：
文献类型：
--
作者：
Komatsu, Takanori;Kobayashi, Toshiya;Kikuchi, Jun
浮游新陈代谢在地球的元素循环中起着至关重要的作用。化学形态和元素化学计量学对于增进我们对浮游生物在生物地球化学循环中的作用的理解是重要的。在这项研究中，利用几种先进的核磁共振技术，提出了一种用于细胞成分化学形态分析的多组分固体核磁共振方法。对鞭毛状原生生物C-13和N-15标记的纤细裸藻进行了单核磁共振测量。三维偶极辅助旋转共振、双交叉极化H-1-C-13相关光谱和H-1-C-13固态异核单量子相关光谱先后实现了细胞成分的表征。然后将这些技术应用于在高铵和低铵条件下培养的纤细乳杆菌，以展示这种方法在分析和比较细胞成分方面的能力。细胞核磁共振谱表明，铵既能诱导副尼龙降解，又能诱导氨化。精氨酸作为氮素储备储存，精氨酸分解代谢通过精氨酸二水解酶途径取代氨氮。N-15和P-31细胞单核磁共振分别显示纤细棘球藻积累精氨酸和聚磷酸盐。通过提供有关环境化学性质的详细信息，这种化学形态形成技术将有助于环境研究。
Planktonic metabolism plays crucial roles in Earth's elemental cycles. Chemical speciation as well as elemental stoichiometry is important for advancing our understanding of planktonic roles in biogeochemical cycles. In this study, a multicomponent solid-state nuclear magnetic resonance (NMR) approach is proposed for chemical speciation of cellular components, using several advanced NMR techniques. Measurements by ssNMR were performed on C-13 and N-15-labeled Euglena gracilis, a flagellated protist. 3D dipolar-assisted rotational resonance, double-cross-polarization H-1-C-13 correlation spectroscopy, and H-1-C-13 solid-state heteronuclear single quantum correlation spectroscopy successively allowed characterization of cellular components. These techniques were then applied to E. gracilis cultured in high and low ammonium media to demonstrate the power of this method for profiling and comparing cellular components. Cellular NMR spectra indicated that ammonium induced both paramylon degradation and amination. Arginine was stored as a nitrogen reserve and ammonium replaced by arginine catabolism via the arginine dihydrolase pathway. N-15 and P-31 cellular ssNMR indicated arginine and polyphosphate accumulation in E. gracilis, respectively. This chemical speciation technique will contribute to environmental research by providing detailed information on environmental chemical properties.