Primary sequence of ionic self-assembling peptide gels affects endothelial cell adhesion and capillary morphogenesis
Primary sequence of ionic self-assembling peptide gels affects endothelial cell adhesion and capillary morphogenesis
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DOI：
10.1002/jbm.a.31785
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Kamm, R. D.
中科院分区：
文献类型：
--
作者：
Sieminski, A. L.;Semino, C. E.;Kamm, R. D.
选择合适的支架材料是体外研究毛细血管形态发生和体内支持组织工程血管化的关键。自组装肽是一类合成的离子型寡肽，当暴露于盐时，其自发地组装成具有ECM样微结构的gets。在本文中，四种不同的自组装肽凝胶促进内皮细胞粘附和毛细血管形态发生的能力进行了探讨。人脐静脉内皮细胞（HUVEC）在离子自组装肽家族成员RAD 16-I（（RADA）（4）、RAD 16-II（（RARADADA）（2））、KFE-8（（FKFE）（2））或KLD-12（（KLDL）（3））中培养。在RAD 16-I或RAD 16-II凝胶中SLI的HUVEC伸长并形成类似于体内毛细血管的互连毛细血管样网络，而在KFE-8或KLD-12凝胶中它们保持圆形并形成簇。由于HUVEC与RAD 16-I和RAD 16-II的粘附明显优于其他肽，这些差异似乎可以通过细胞粘附的差异来解释。虽然粘附可能通过结合的粘附蛋白发生，但与所研究的肽的蛋白结合似乎没有差异。结果表明，虽然这些寡肽具有相似的自组装机制，但它们的一级序列可以极大地影响细胞粘附。此外，这些仿生ECM样材料的子集支持毛细血管形态发生，因此可能有助于支持血管形成。(C)2008 Wiley Periodicals，Inc.
Appropriate choice of biomaterial Supports is critical for the study of capillary morphogenesis in vitro as well as to support vascularization of engineered tissues in vivo. Self-assembling peptides are a class of synthetic, ionic, oligopeptides that spontaneously assemble into gets with an ECM-like microarchitecture when exposed to salt. In this paper, the ability of four different self-assembling peptide gels to promote endothelial cell adhesion and capillary morphogenesis is explored. Human umbilical vein endothelial cells (HUVECs) were cultured within ionic self-assembling peptide family members, RAD16-I ((RADA)(4), RAD16-II ((RARADADA)(2)), KFE-8 ((FKFE)(2)), or KLD-12 ((KLDL)(3)). HUVECs SLIspended in RAD16-I or RAD16-II gels elongated and formed interconnected capillary-like networks resembling in vivo capillaries, while they remained round and formed clusters within KFE-8 Or KLD-12 gels. As HUVECs attach to RAD16-I and RAD16-II significantly better than the other peptides, these differences appear to be explained by differences in cell adhesion. Although adhesion likely occurs via bound adhesion proteins, there appears to be no difference in protein binding to the peptides investigated. Results indicate that, although these oligopeptides have similar mechanisms of self-assembly, their primary sequence can greatly affect cell adhesion. Additionally, a Subset of these biomimetic ECM-like materials Support capillary morphogenesis and thus may be useful for supporting vascularization. (C) 2008 Wiley Periodicals, Inc.