Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, interacts with factors of both the 60 S and the 40 S ribosomal subunit assembly
Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, interacts with factors of both the 60 S and the 40 S ribosomal subunit assembly
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DOI：
10.1074/jbc.m403338200
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发表时间：
2004-06-11
影响因子：
4.8
通讯作者：
Mizuta, K
中科院分区：
文献类型：
--
作者：
Shirai, C;Takai, T;Mizuta, K
Ebp 2 p是人EB病毒核抗原1结合蛋白2的酵母同源物，对60 S核糖体亚基的生物合成至关重要。双杂交筛选显示，除了60 S亚基组装所需的因子外，Ebp 2 p还与Rps 16 p、核糖体蛋白S16和40 S核糖体亚基组装因子Utp 11 p以及Yil 019 w相互作用，其功能以前未被表征。Yil 019 w的缺失导致18 S rRNA和40 S核糖体亚基的水平降低，而不影响25 S rRNA和60 S核糖体亚基的水平。35 S前体rRNA和异常23 S RNA积累，表明当Yil 019 w耗尽时，位点A（0）-A（2）处的前体rRNA加工被抑制。来自Yil 019 w、Utp 11 p和Rps 16 p的每个组合都表现出双杂交互作。
Ebp2p, the yeast homolog of human Epstein-Barr virus nuclear antigen 1-binding protein 2, is essential for biogenesis of the 60 S ribosomal subunit. Two-hybrid screening exhibited that, in addition to factors necessary for assembly of the 60 S subunit, Ebp2p interacts with Rps16p, ribosomal protein S16, and the 40 S ribosomal subunit assembly factor, Utp11p, as well as Yil019w, the function of which was previously uncharacterized. Depletion of Yil019w resulted in reduction in levels of both of 18 S rRNA and 40 S ribosomal subunit without affecting levels of 25 S rRNA and 60 S ribosomal subunits. 35 S pre-rRNA and aberrant 23 S RNA accumulated, indicating that pre-rRNA processing at sites A(0)-A(2) is inhibited when Yil019w is depleted. Each combination from Yil019w, Utp11p, and Rps16p showed two-hybrid interaction.