Nitrogen flow and microbial community in the anoxic reactor of "Sulfate Reduction, Denitrification/Anammox and Partial Nitrification" process
Nitrogen flow and microbial community in the anoxic reactor of "Sulfate Reduction, Denitrification/Anammox and Partial Nitrification" process
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DOI：
10.1016/j.bej.2019.107304
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发表时间：
2019-11-15
影响因子：
3.9
通讯作者：
Yamamoto-Ikemoto, Ryoko
中科院分区：
文献类型：
--
作者：
Kosugi, Yuka;Matsuura, Norihisa;Yamamoto-Ikemoto, Ryoko
采用硫酸盐还原-反硝化/厌氧氨氧化-短程硝化工艺处理有机废水。采用厌氧-缺氧生物滤池反应器考察了该工艺中缺氧反应器的脱氮效果。在模拟硝化反应器回流的缺氧反应器中加入硝酸盐介质，主要发生异养反硝化和硫反硝化。当亚硝酸盐进入模拟部分硝化水的缺氧反应器中时，反应器中同时发生了厌氧氨氧化和反硝化。厌氧氨氧化反应对亚硝酸盐的去除率达到30%以上。通过16 S rRNA基因测序分析，证实了缺氧反应器中异养反硝化细菌和硫氧化细菌共存，但各反应器中异养反硝化细菌和硫氧化细菌的群落结构不同。在亚硝酸盐进料的反应器中检测到候选人Brocadia，这意味着即使在高硫化物条件下，厌氧氨氧化细菌也与反硝化细菌共存。
We propose a sulfate reduction, denitrification/anammox and partial nitrification process to treat organic wastewater. Nitrogen removal in the anoxic reactor in this process was examined using an anaerobic-anoxic biofilter reactor. When nitrate medium was added to the anoxic reactor simulating the return flow from a nitrification reactor, mainly heterotrophic and sulfur denitrification occurred. However, anammox as well as denitrification occurred in the anoxic reactor when nitrite was fed into the reactor simulating partially nitrified water. The proportion of nitrite removal by the anammox reaction reached over 30%. By 16S rRNA gene sequencing analysis, the coexistence of heterotrophic and sulfur denitrification bacteria was confirmed in the anoxic reactor, although the heterotrophic denitrification and sulfur oxidation bacterial communities were different in each reactor. Candidates Brocadia was detected in the nitrite-fed reactor, meaning that anammox bacteria coexist with denitrification bacteria even in high-sulfide conditions.