Loss of calretinin- and parvalbumin-immunoreactive axons in anterolateral columns beyond the corticospinal tracts of amyotrophic lateral sclerosis spinal cords
Loss of calretinin- and parvalbumin-immunoreactive axons in anterolateral columns beyond the corticospinal tracts of amyotrophic lateral sclerosis spinal cords
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DOI：
10.1016/j.jns.2013.05.008
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发表时间：
2013-08-15
影响因子：
4.4
通讯作者：
Okamoto, Koichi
中科院分区：
文献类型：
--
作者：
Hayashi, Shintaro;Amari, Masakuni;Okamoto, Koichi
在肌萎缩侧索硬化症（ALS）的脊髓，弥漫性髓鞘苍白（dMP）的前外侧柱（ALCs）超出皮质脊髓束已被频繁观察到，然而，其起源仍有待阐明。为了解决这个问题，我们重点研究了钙视网膜蛋白（CR）和小清蛋白（PV），因为这些缓冲钙结合蛋白（CaBP）主要在神经正常的人类脊髓白色物质的ALC的轴突中表达。免疫组化结果显示ALS患者dMP组脊髓中CR免疫反应阳性（ir）和PV免疫反应阳性（ir）轴突的数量均显著低于对照组。在没有dMP的ALS患者的脊髓中，与对照组相比，这些CaBP-ir轴突的数量也显著减少。相反，脊髓灰质中CR-IR神经元的数量在ALS患者和对照组之间没有显着差异。这些结果表明，在ALS患者的脊髓中，CaBP-ir轴突的损失可能先于dMP的发展，并且死亡回机制可能是这种现象的基础。(C)2013爱思唯尔有限公司版权所有。
In amyotrophic lateral sclerosis (ALS) spinal cords, diffuse myelin pallor (dMP) in the anterolateral columns (ALCs) beyond the corticospinal tracts has been frequently observed; however, its origin still remains to be elucidated. To address this issue, we focused on calretinin (CR) and parvalbumin (PV), since these buffer calcium-binding proteins (CaBP) are predominantly expressed in axons in the ALCs of neurologically normal human spinal white matter. Immunohistochemical methods revealed that numbers of both CR-immunoreactive (ir) and PV-ir axons were significantly lower in ALS patients' spinal cords with dMP compared to those in controls. In ALS patients' spinal cords without dMP, there were also significant reductions in the number of these CaBP-ir axons compared to controls. In contrast the number of CR-ir neurons in the spinal gray matter did not differ significantly among ALS patients and controls. These findings suggest that a loss of CaBP-ir axons may precede the development of dMP in ALS patients' spinal cords, and the dying back mechanism would underlie this phenomenon. (C) 2013 Elsevier B.V. All rights reserved.