Quantitative analysis of leukocyte membrane antigen expression on human fetal and cord blood: Normal values and changes during development
Quantitative analysis of leukocyte membrane antigen expression on human fetal and cord blood: Normal values and changes during development
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DOI：
10.1006/clin.1997.4366
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发表时间：
1997-07-01
期刊：
影响因子：
--
通讯作者：
Sampol, J
中科院分区：
文献类型：
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作者：
Bikoue, A;DErcole, C;Sampol, J
我们研究了正常人胎儿和足月新生儿的各种细胞表面抗原的抗体结合能力（ABC）的淋巴细胞，单核细胞和多形核细胞（PMN）细胞的定量细胞术也指定的定量。分析了这些白细胞在发育过程中表达水平的变化。结果表明，大多数标记的ABC值在成熟过程中发生变化。研究的淋巴细胞相关抗原如CD 5和CD 7的ABC仅显示从胎儿到成人的降低，而根据单核细胞和PMN上的分子类型，从胎儿到新生儿或从新生儿到成人的发育过程的阶段，ABC值增加或减少。然而，所有白细胞上的白细胞膜抗原（例如CD 16、CD 46和CD 55）以及骨髓细胞上的CD 11b、CD 11 c和CD 35的ABC值没有变化。与成体细胞相比，它们在胎儿中的表达水平已经成熟。此外，在这种定量方法中，对CD 11 a和CD 8结果的分析表明，CD 11 a在淋巴细胞亚群上表达水平的变化可能取决于一种机制，而CDS可能至少有两种机制。此外，CD 5、CD 7和CD 11 a的表达模式在成熟过程中发生变化。我们的结论是，即使新生儿的免疫应答模式不同于成人，这主要是基于淋巴细胞T亚群（特别是TH 1/TH 2）的相对数量和功能活性及其细胞因子谱，这些定量和定性的表型差异也可能有助于解释白细胞胎儿和脐带血细胞的功能特性。所有这些发现支持ABC表达的不成熟和成熟的概念。(C)北京：科学出版社.
We studied the antibody binding capacity (ABC) of various cell-surface antigens in normal human fetuses and term neonates on lymphocyte, monocyte, and polymorphonuclear (PMN) cells by quantitative how cytometry also designated by quantimetry. Analysis of changes of expression level on these leukocytes during the developmental process was also investigated. The results indicated that the ABC values of most studied markers change during the maturational process. The ABC of lymphocyte-associated antigens studied such as CD5 and CD7 showed only a decrease from fetus to adult, whereas according to the type of molecule on monocyte and PMN there was either an increase or a decrease of ABC values dependent on the stage of the developmental process, from fetus to neonate or from neonate to adult. However, the ABC values of leukocyte membrane antigens such as CD16, CD46, and CD55 on all leukocytes and CD11b, CD11c, and CD35 on myeloid cells did not change. Their expression level was already mature in fetuses compared with adult cells. In addition, in this quantimetric approach, the analysis of the results for CD11a and CD8 suggested that the changes of CD11a expression level on lymphocyte subsets can depend on one mechanism, whereas there are probably at least two for CDS. Furthermore, the expression patterns of CD5, CD7, and CD11a change during maturation. We concluded that, even if the neonate response pattern to immunological challenge differs from an adult and this is based primarily on the relative numbers and functional activity of lymphocyte T subsets (especially TH1/TH2) and their cytokine profiles, these quantitative and qualitative phenotypical differences might also contribute to explain the functional peculiarities of leukocyte fetal and cord blood cells. All these findings support the notion of immaturity and maturity of ABC expression. (C) 1997 Academic Press.