MiR-125b Suppression Inhibits Apoptosis and Negatively Regulates Sema4D in Avian Leukosis Virus-Transformed Cells
MiR-125b Suppression Inhibits Apoptosis and Negatively Regulates Sema4D in Avian Leukosis Virus-Transformed Cells
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MiR-125b 抑制可抑制禽白血病病毒转化细胞中的细胞凋亡并负调节 Sema4D
DOI：
10.3390/v11080728
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发表时间：
2019-08
期刊：
影响因子：
4.7
通讯作者：
Gao Yulong
中科院分区：
文献类型：
--
作者：
Ren Chaoqi;Xie Ruyu;Yao Yongxiu;Yu Mengmeng;Chang Fangfang;Xing Lixiao;Zhang Yao;Liu Yongzhen;Wang Suyan;Farooque Muhammad;Wang Yongqiang;Qi Xiaole;Liu Changjun;Zhang Yanping;Cui Hongyu;Li Kai;Gao Li;Pan Qing;Nair Venugopal;Wang Xiaomei;Gao Yulong
Subgroup J avian leukosis virus (ALV-J), an oncogenic retrovirus, causes hemangiomas and myeloid tumors in chickens. We previously showed that miR-125b is down-regulated in ALV-J-induced tumors. This study aimed to investigate the possible role of miR-125b in ALV-J-mediated infection and tumorigenesis. Knockdown of miR-125b expression in HP45 cells reduced, whereas over-expression induced late-stage apoptosis. Bioinformatics analysis and luciferase activity assays indicate that miR-125b targets Semaphorin 4D/CD100 (Sema4D) by binding the 3′-untranslated region of messenger RNA (mRNA). Up-regulation of miR-125b in the DF1 cell line suppressed Sema4D expression, whereas miR-125 down-regulation increased Sema4D expression levels. To uncover the function of Sema4D during ALV-J infection, animal infection experiments and in vitro assays were performed and show that Sema4D mRNA levels were up-regulated in ALV-J-infected tissues and cells. Finally, functional experiments show that miR-125 down-regulation and Sema4D over-expression inhibited apoptosis in HP45 cells. These results suggest that miR-125b and its target Sema4D might play an important role in the aggressive growth of HP45 cells induced by avian leukosis viruses (ALVs). These findings improve our understanding of the underlying mechanism of ALV-J infection and tumorigenesis.
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影响因子：
2.9
作者：
Yilong Yao;Anwaier Reheman;Yefei Xu;Qifa Li
通讯作者：
Yilong Yao;Anwaier Reheman;Yefei Xu;Qifa Li
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影响因子：
3.3
作者：
Qin, Liting;Gao, Honglei;Wang, Yongqiang;Wang, Xiaomei
通讯作者：
Wang, Xiaomei
影响因子：
2.4
作者：
S. Astrin;P. Rothberg
通讯作者：
S. Astrin;P. Rothberg
影响因子：
6.4
作者：
Huang, Shenglin;He, Xianghuo;Gu, Jianren
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Gu, Jianren
贾亚型禽白血病病毒感染过程中 DF-1™ 细胞体内 miRNA 表达失调
影响因子：
3.8
作者：
Jun Ji;Xin Xu;Xueyu Wang;Lunguang Yao;Huiqin Shang;Hongxin Li;Jingyun Ma;Yingzuo Bi;Qingmei Xie
通讯作者：
Qingmei Xie