Glucosidation of betulinic acid by Cunninghamella species
Glucosidation of betulinic acid by Cunninghamella species
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DOI：
10.1021/np980432b
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发表时间：
1999-05-01
影响因子：
5.1
通讯作者：
Kouzi, SA
中科院分区：
文献类型：
--
作者：
Chatterjee, P;Pezzuto, JM;Kouzi, SA
研究了抗黑素瘤剂白桦酸(1)的微生物转化。利用Cunninghamella物种NRRL 5695的静息细胞悬悬液进行制备规模生物转化，产生真菌代谢产物1，根据光谱和酶学数据表征为28-o - β - d -glucopyranosyl 3 - β -羟基- lupp -20(29)-en-28-oate(2)。体外细胞毒性试验显示，代谢物2对几种人类黑色素瘤细胞系无活性。
Microbial transformation of the antimelanoma agent betulinic acid (1) was studied. Preparative scale biotransformation with resting-cell suspensions of Cunninghamella species NRRL 5695 resulted in the production of a fungal metabolite of 1, which has been characterized as 28-O-beta-D-glucopyranosyl 3 beta-hydroxy-lup-20(29)-en-28-oate (2) based on spectral and enzymic data. The in vitro cytotoxicity assay of metabolite 2 revealed no activity against several human melanoma cell lines.