Astaxanthin ameliorates cartilage damage in experimental osteoarthritis
Astaxanthin ameliorates cartilage damage in experimental osteoarthritis
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DOI：
10.3109/14397595.2015.1008724
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发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Chen, Wei-Ping
中科院分区：
文献类型：
--
作者：
Huang, Li-Juan;Chen, Wei-Ping
目的.虾青素是在某些海洋动物和植物中发现的红色色素类胡萝卜素。体外研究表明，虾青素可抑制基质金属蛋白酶（MMPs）的表达。然而，虾青素对软骨的影响仍不清楚。本研究旨在探讨虾青素对实验性骨关节炎（OA）软骨的影响。新西兰兔行前交叉韧带切断术，诱导右膝OA。兔接受含有0.3 ml载体（二甲基亚砜）或虾青素（50 μ M）的关节内注射。于手术当天开始注射，每周注射一次，连续6周。然后，处死家兔，取右膝进行研究。通过形态学和组织学检查评估，虾青素减少了胡萝卜素的降解。与溶剂组相比，虾青素可抑制软骨中MMP-1、MMP-3和MMP-13的基因表达。结果表明，虾青素可能被认为是治疗OA的药物制剂。
Purpose. Astaxanthin is a red-pigment carotenoid found in certain marine animals and plants. Astaxanthin has been shown to inhibit matrix metalloproteinases (MMPs) expression in vitro. However, the effect of astaxanthin on cartilage is still unclear. The aim of this study was to investigate the effects of astaxanthin on cartilage in experimental osteoarthritis (OA).Methods. New Zealand rabbits underwent anterior cruciate ligament transection to induce OA in right knee. Rabbits received intra-articular injection containing 0.3 ml of vehicle (dimethyl sulfoxide) or astaxanthin (50 mu M). Injection was started on the day of operation, and the injection were performed once weekly for six consecutive weeks. Then, rabbits were sacrificed and the right knees were harvested for study.Results. Cartilage degradation was reduced by astaxanthin, as assessed by morphological and histological examination. Astaxanthin inhibited the gene expression of MMP-1, MMP-3, and MMP-13 in cartilage as compared with the vehicle group.Conclusions. The results suggest that astaxanthin may be considered as pharmaceutical agent in OA treatment.