Organization and expression of the left third of the genome of adenovirus.
Organization and expression of the left third of the genome of adenovirus.
复制标题
腺病毒基因组左三分之一的组织和表达。
DOI：
10.1101/sqb.1980.044.01.052
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
M. Mathews
中科院分区：
文献类型：
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作者：
J. B. Lewis;H. Esche;J. Smart;B. Stillman;M. Harter;M. Mathews
人类腺病毒基因组中与肿瘤形成过程相关的区域已经使用几种方法进行了鉴定。对病毒转化的不同细胞系中包含的病毒DNA的分析表明，保留基因组最左侧的14%足以维持这些细胞转化后的生长特性(Gallimore等人)。1974年)。在转化的细胞系中表达的腺病毒mRNAs类似于在生产性感染的早期阶段从这些DNA序列中表达的mRNAs(Flint等人。1975)。病毒DNA的左端至少包含两个转化所必需的基因，因为在这一区域内定位的两组宿主范围突变体的互补组(Frost和Williams 1978)都有转化缺陷(Graham等人)。1978年)。用病毒DNA片段转染细胞提供了一种直接确定病毒最低含量的方法.
The regions of the human adenoviral genome associated with the process of oncogenesis have been identified using several approaches. Analysis of the viral DNAs contained in different lines of cells transformed by virus has demonstrated that retention of the leftmost 14% of the genome is sufficient for the maintenance of the transformed growth properties of these cells (Gallimore et al. 1974). The adenoviral mRNAs expressed in transformed cell lines are similar to those expressed from these DNA sequences during the early phase of the productive infection (Flint et al. 1975). The left end of the viral DNA contains at least two genes necessary for transformation, since two complementation groups of host-range mutants that map within this region (Frost and Williams 1978) are both defective for transformation (Graham et al. 1978). Transfection of cells with fragments of viral DNA has provided a direct means of determining the minimum amount of viral...