Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea.
Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea.
复制标题
DOI：
10.1186/1471-213x-11-69
复制
发表时间：
2011-11-10
影响因子：
--
通讯作者：
Newmark PA
中科院分区：
文献类型：
--
作者：
Chong T;Stary JM;Wang Y;Newmark PA
淡水真涡虫Schmidtea meditalienea表现出两种不同的生殖模式。有性品系的个体是具有胚胎后发育的生殖器官的交叉雌雄同体。相反，无性系的个体只通过横向分裂繁殖，不能发育生殖器官。这些不同的繁殖菌株与不同的核型有关，使S。medialea是研究生殖系发育和性别分化的有用模型。为了鉴定这些菌株之间差异表达的基因，我们进行了微阵列分析，并鉴定了>800个在有性涡虫中上调的基因。从这些，我们的特点是24个基因的荧光原位杂交（FISH），揭示其在男性生殖细胞或附属生殖器官的表达。为了确定其他标志物的涡虫生殖系统，我们还使用免疫和荧光凝集素染色，确定几种抗体和凝集素，标记与生殖器官相关的结构。总的来说，这些细胞类型的特异性标记将使未来的努力，以表征基因是重要的生殖发育的真涡虫。
The freshwater planarian Schmidtea mediterranea exhibits two distinct reproductive modes. Individuals of the sexual strain are cross-fertilizing hermaphrodites with reproductive organs that develop post-embryonically. By contrast, individuals of the asexual strain reproduce exclusively by transverse fission and fail to develop reproductive organs. These different reproductive strains are associated with distinct karyotypes, making S. mediterranea a useful model for studying germline development and sexual differentiation. To identify genes expressed differentially between these strains, we performed microarray analyses and identified >800 genes that were upregulated in the sexual planarian. From these, we characterized 24 genes by fluorescent in situ hybridization (FISH), revealing their expression in male germ cells or accessory reproductive organs. To identify additional markers of the planarian reproductive system, we also used immuno- and fluorescent lectin staining, identifying several antibodies and lectins that labeled structures associated with reproductive organs. Collectively, these cell-type specific markers will enable future efforts to characterize genes that are important for reproductive development in the planarian.