Involvement of BcYak1 in the Regulation of Vegetative Differentiation and Adaptation to Oxidative Stress of Botrytis cinerea.
Involvement of BcYak1 in the Regulation of Vegetative Differentiation and Adaptation to Oxidative Stress of Botrytis cinerea.
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BcYak1参与灰葡萄孢营养分化和氧化应激适应的调节
DOI：
10.3389/fmicb.2018.00281
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发表时间：
2018
影响因子：
5.2
通讯作者：
Liang W
中科院分区：
文献类型：
--
作者：
Yang Q;Zhang J;Hu J;Wang X;Lv B;Liang W
Yak 1是一种双特异性酪氨酸磷酸化调节蛋白激酶，在真菌的多种细胞过程中发挥重要作用。然而，到目前为止，BcYak 1在灰葡萄孢（Botrytis cinerea）中的作用，灰霉病在各种植物物种中的致病因子，仍然没有表征。我们先前的研究确定了一个赖氨酸乙酰化位点（Lys 252）在BcYak 1，这是第一次报告这样的网站在Yak 1。本研究采用基因破坏和定点突变的方法对BcYak 1及其赖氨酸乙酰化位点的功能进行了研究。该基因缺失突变体Δ BcYak 1不仅致病性、产孢和菌核形成能力显著降低，而且对H2 O2和麦角甾醇生物合成抑制剂（EBI）三唑酮的敏感性显著提高。然而，Lys 252定点突变菌株Δ BcYak 1-K252 Q（模拟该位点的乙酰化）仅显示出较低的菌核形成和较高的H2 O2敏感性。这些结果表明，BcYAK 1基因参与了B的营养分化、对氧化胁迫和三唑酮的适应以及毒力的调节。灰绿色
Yak1, a member of the dual-specificity tyrosine phosphorylation-regulated protein kinases, plays an important role in diverse cellular processes in fungi. However, to date, the role of BcYak1 in Botrytis cinerea, the causal agent of gray mold diseases in various plant species, remains uncharacterized. Our previous study identified one lysine acetylation site (Lys252) in BcYak1, which is the first report of such a site in Yak1. In this study, the function of BcYak1 and its lysine acetylation site were investigated using gene disruption and site-directed mutagenesis. The gene deletion mutant ΔBcYak1 not only exhibits much lower pathogenicity, conidiation and sclerotium formation, but was also much more sensitive to H2O2 and the ergosterol biosynthesis inhibitor (EBI) triadimefon. The Lys252 site-directed mutagenesis mutant strain ΔBcYak1-K252Q (mimicking the acetylation of the site), however, only showed lower sclerotium formation and higher sensitivity to H2O2. These results indicate that BcYAK1 is involved in the vegetative differentiation, adaptation to oxidative stress and triadimefon, and virulence of B. cinerea.