Formation and spreading of lipid bilayers on planar glass supports
Formation and spreading of lipid bilayers on planar glass supports
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DOI：
10.1021/jp983996x
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发表时间：
1999-04-01
影响因子：
3.3
通讯作者：
Boxer, SG
中科院分区：
文献类型：
--
作者：
Cremer, PS;Boxer, SG
研究了玻璃表面磷脂双层的融合和铺展与 pH 和离子强度的关系。对于含有净负电荷的囊泡，在高离子强度和低 pH 条件下，膜与载体的融合是有利的；然而，中性和带正电的囊泡在所有尝试的条件下都会融合。这一结果表明范德华力和静电相互作用控制着聚变过程。无论双层上的净电荷如何，膜在平面表面上的铺展在低pH值下是有利的，并且该过程是由范德华力驱动的。另一方面，在高 pH 值或高度弯曲的表面上，膜的传播会受到阻碍。在这种情况下，水合和弯曲相互作用的组合主要负责阻止扩散过程。这些结果为理解影响平面支撑双层上划痕作为横向扩散屏障的有效性的许多因素提供了一个框架，并产生了一种修复这些划痕的简单方法。
The fusion and spreading of phospholipid bilayers on glass surfaces was investigated as a function of pH and ionic strength. Membrane fusion to the support was favorable at high ionic strength and low pH for vesicles containing a net negative charge; however, neutral and positively charged vesicles fused under all conditions attempted. This result suggests that van der Waals and electrostatic interactions govern the fusion process. Membrane spreading over a planar surface was favorable at low pH regardless of the net charge on the bilayer, and the process is driven by van der Waals forces. On the other hand membrane propagation is impeded at high pH or on highly curved surfaces. In this case a combination of hydration and bending interactions is primarily responsible for arresting the spreading process. These results provide a framework for understanding many of the factors that influence the effectiveness of scratches on planar supported bilayers as barriers to lateral diffusion and lead to a simple method to heal these scratches.