Cryptochromes suppress leaf senescence in response to blue light in Arabidopsis
Cryptochromes suppress leaf senescence in response to blue light in Arabidopsis
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DOI：
10.1093/plphys/kiad042
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发表时间：
2023-01-30
期刊：
影响因子：
7.4
通讯作者：
Kusaba, Makoto
中科院分区：
文献类型：
--
作者：
Kozuka, Toshiaki;Oka, Yoshito;Kusaba, Makoto
蓝光光感受器隐花色素通过调控HY 5和PIF 4/5转录因子来抑制叶片对蓝光的响应，从而有效地改变叶片衰老的诱导和进程。当植物生长在邻近植被的浓密阴影下时，叶片衰老反应增强。虽然光谱中红色和蓝色区域的通量率在阴影下强烈衰减，但支持蓝光反应的感光机制仍然不清楚。在这项研究中，我们分析了叶片衰老响应蓝光在拟南芥（拟南芥）。我们发现，叶片衰老促进消除活性光敏色素PFR脉冲远红（FR）光，而蓝光照射抑制叶片衰老的野生型，但不是在隐花色素（CRY）缺陷的突变体，cry 1 cry 2。因此，两种光感应模式有助于抑制叶片衰老，这取决于光谱特征。首先是叶片衰老响应蓝光，这是介导的隐花色素。其次是光敏色素介导的叶片衰老响应红色/FR光。转基因植物表达绿色荧光蛋白（GFP）-标记的p53 2的生理分析表明，光激活的隐花色素需要抑制叶片衰老响应蓝光。转录组学分析进一步揭示了隐花色素下游参与调控叶片衰老的分子和细胞过程。此外，隐花色素下游成分的分析表明，伸长下胚轴5（HY 5）和光敏色素相互作用因子（PIF）4和PIF 5所需的抑制和促进叶片衰老，分别。
Blue light photoreceptors, cryptochromes, suppress the progression of leaf senescence in response to blue light through the regulation of HY5 and PIF4/5 transcriptional factors.The induction and progression of leaf senescence are effectively changed according to the light environment. The leaf senescence response is enhanced when plants are grown under a dense shade cast by neighboring vegetation. Although the fluence rate of the red and blue regions in the light spectrum is strongly attenuated under shade, photosensory mechanisms that underpin the blue light response are still unclear. In this study, we analyzed leaf senescence in response to blue light in Arabidopsis (Arabidopsis thaliana). We found that leaf senescence was promoted by the elimination of active phytochrome Pfr by pulsed far-red (FR) light, whereas irradiation with blue light suppressed leaf senescence in the wild type but not in the cryptochrome (CRY)-deficient mutant, cry1 cry2. Hence, two light-sensing modes contributed to the suppression of leaf senescence that was dependent on light spectrum features. First was the leaf senescence response to blue light, which was mediated exclusively by cryptochromes. Second was the phytochrome-mediated leaf senescence response to red/FR light. Physiological analysis of transgenic plants expressing green fluorescent protein (GFP)-tagged CRY2 revealed that photo-activation of cryptochromes was required to suppress leaf senescence in response to blue light. Transcriptomic analysis further uncovered the molecular and cellular processes involved in the regulation of leaf senescence downstream of cryptochromes. Furthermore, analysis of cryptochrome-downstream components indicated that ELONGATED HYPOCOTYL 5 (HY5) and PHYTOCHROME INTERACTING FACTOR (PIF) 4 and PIF5 were required for suppression and promotion of leaf senescence, respectively.