Differences in the response to periarterial nerve stimulation or exogenous noradrenaline infusion in the mesenteric vascular bed with the intestinal tract harvested from commonly used rat models of hypertension
Differences in the response to periarterial nerve stimulation or exogenous noradrenaline infusion in the mesenteric vascular bed with the intestinal tract harvested from commonly used rat models of hypertension
复制标题
肠系膜血管床对动脉周围神经刺激或外源性去甲肾上腺素输注与常用高血压大鼠模型中采集的肠道的反应差异
DOI：
10.1111/1440-1681.13068
复制
发表时间：
2019
影响因子：
2.9
通讯作者：
Yamamoto R.
中科院分区：
文献类型：
--
作者：
Gamoh S; Shiba T; DiPette DJ; Yamamoto R.
许多高血压动物模型已被开发出来，并用于阐明高血压的病理生理学和开发抗高血压药物。其中，常用的有自发性高血压大鼠（SHR）、醋酸脱氧皮质酮（DOCA）处理的高盐摄入大鼠（DOCA-盐）、高钠喂养的Dahl盐敏感大鼠（HS）模型。已经进行了多项研究，然而，在这些模型中由于肾上腺素能血管收缩的反应性引起的血压升高尚未在集中实验中得到很好的表征。本研究在离体肠系膜血管床上，通过测量动脉周围神经刺激（PNS）或外源性去甲肾上腺素（NA）灌注引起的升压反应，探讨肠系膜肾上腺素能血管收缩反应性。高血压大鼠模型的体循环动脉血压与其各自的对照组相比均匀升高。然而，在肠系膜血管床中的灌注压的变化响应于PNS和外源性NA输注是完全不同的，这取决于模型。SHR和Dahl S HS大鼠对PNS的升压反应显著高于对照组，DOCA-盐大鼠对PNS的升压反应显著低于对照组。SHR对外源性NA的升压反应明显高于对照组，而Dahl S HS大鼠的升压反应明显低于对照组。DOCA-盐组和假手术组对外源性NA的升压反应无差异。结果表明，不同类型的实验性高血压大鼠肾上腺素能血管收缩反应性不同。
Many hypertensive animal models have been developed and used to elucidate the pathophysiology of hypertension and to develop antihypertensive drugs. Among them, the spontaneous hypertensive rat (SHR), deoxycorticosterone acetate (DOCA)‐treated and high salt intake rat (DOCA‐salt), and high sodium‐fed Dahl salt‐sensitive rat (HS) models are commonly used. Multiple studies have been conducted, however, elevation in blood pressure in these models due to the reactivity of adrenergic vasoconstriction has not been well characterized in a centralized experiment. In this study, the pressor responses to periarterial nerve stimulation (PNS) or exogenous noradrenaline (NA) infusion were measured in the isolated mesenteric vascular bed with the intestinal tract to investigate the reactivity of mesenteric adrenergic vasoconstriction. The systemic arterial blood pressure of the hypertensive rat models was uniformly elevated compared with their respective controls. However, the changes in perfusion pressure in the mesenteric vascular bed in response to PNS and exogenous NA infusion were quite different depending on the model. The pressor responses to PNS in SHRs and Dahl S HS rats were significantly higher, and those in DOCA‐salt rats were significantly lower than those in the controls. The pressor responses to exogenous NA infusion in SHRs were significantly higher, and those in Dahl S HS rats were significantly lower than those in their respective controls. No difference was observed in the pressor responses to the exogenous NA between the DOCA‐salt and sham groups. These results demonstrate that the reactivity of adrenergic vasoconstriction is different for each type of experimental hypertensive model rat.