ABNORMAL PROTEINS SERVE AS EUKARYOTIC STRESS SIGNALS AND TRIGGER THE ACTIVATION OF HEAT-SHOCK GENES
ABNORMAL PROTEINS SERVE AS EUKARYOTIC STRESS SIGNALS AND TRIGGER THE ACTIVATION OF HEAT-SHOCK GENES
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DOI：
10.1126/science.3083508
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发表时间：
1986-04-25
期刊：
影响因子：
56.9
通讯作者：
VOELLMY, R
中科院分区：
文献类型：
--
作者：
ANANTHAN, J;GOLDBERG, AL;VOELLMY, R
热休克蛋白(HSP)基因是一组普遍存在的基因，在各种代谢应激条件下被激活。通过将纯化的蛋白质和HSP基因共同注射到非洲爪蛙卵母细胞中，验证了细胞内蛋白质的变性可能是由于代谢应激而产生的，然后发出了HSP基因激活的信号。如果蛋白质在注射前变性，可以观察到HSP基因的激活，但如果它们是以天然形式引入的，则不会观察到激活。此外，异常蛋白和热休克激活HSP基因似乎是通过共同的机制发生的。基因转录调控的模型是基于异常细胞内蛋白和不稳定的调控因子之间的降解竞争。
Heat shock protein (hsp) genes, a group of ubiquitous genes, are activated by various metabolic stresses. The suggestion that denaturation of intracellular proteins may be produced by the metabolic stresses and then signal the activation of the hsp genes was examined by co-injection of purified proteins and hsp genes into frog [Xenopus laevis] oocytes. Activation of hsp genes was observed if the proteins were denatured prior to injection but not if they were introduced in their native form. Furthermore, the activation of hsp genes by abnormal proteins and by heat shock appears to occur by a common mechanism. A model for the transcriptional regulation of the genes is based on competition for degradation between abnormal intracellular proteins and a labile regulatory factor.