MPK3-and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis
MPK3-and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis
复制标题
MPK3 和 MPK6 介导的 ICE1 磷酸化负向调节拟南芥中 ICE1 的稳定性和冷冻耐受性
DOI：
10.1016/j.devcel.2017.09.025
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发表时间：
2017-12-04
影响因子：
11.8
通讯作者：
Yang, Shuhua
中科院分区：
文献类型：
--
作者：
Li, Hui;Ding, Yanglin;Yang, Shuhua
低温影响植物生长、发育、生产力和生态分布。冷胁迫诱导了C-重复序列结合因子（CBF）转录因子的表达，进而激活了获得抗冻性所需的下游冷响应（COR）基因。CBF表达诱导因子1（ICE 1）是CBF的主要调节因子，ICE 1的稳定性对其功能至关重要。然而，ICE 1的调节并不很清楚。在这里，我们报告了丝裂原活化蛋白激酶3（MPK 3）和MPK 6与ICE 1相互作用并使其磷酸化，从而降低其稳定性和转录活性。一致地，mpk 3和mpk 6单突变体和mpk 3/mpk 6双突变体显示增强的抗冻性，而MPK 3/MPK 6激活减弱抗冻性。ICE 1 -2突变体中ICE 1补体冷冻敏感性的磷酸化失活突变这些组合的结果表明，MPK 3/MPK 6磷酸化和不稳定的ICE 1，负调控CBF的表达和植物的抗冻性。
Low temperatures affect plant growth, development, productivity, and ecological distribution. Expression of the C-repeat-binding factor (CBF) transcription factors is induced by cold stress, which in turn activates downstream cold-responsive (COR) genes that are required for the acquisition of freezing tolerance. Inducer of CBF expression 1 (ICE1) is a master regulator of CBFs, and ICE1 stability is crucial for its function. However, the regulation of ICE1 is not well understood. Here, we report that mitogen-activated protein kinase 3 (MPK3) and MPK6 interact with and phosphorylate ICE1, which reduces its stability and transcriptional activity. Consistently, the mpk3 and mpk6 single mutants and the mpk3 mpk6 double mutants show enhanced freezing tolerance, whereas MPK3/MPK6 activation attenuates freezing tolerance. Phosphor-inactive mutations of ICE1 complement freezing sensitivity in the ice1-2 mutant. These combined results indicate that MPK3/MPK6 phosphorylate and destabilize ICE1, which negatively regulates CBF expression and freezing tolerance in plants.