ENHANCED ASPARTATE RELEASE FROM HIPPOCAMPAL SLICES OF EPILEPTIC (EL) MICE
ENHANCED ASPARTATE RELEASE FROM HIPPOCAMPAL SLICES OF EPILEPTIC (EL) MICE
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DOI：
10.1111/j.1471-4159.1991.tb02021.x
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发表时间：
1991-03-01
影响因子：
4.7
通讯作者：
SEYFRIED, TN
中科院分区：
文献类型：
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作者：
FLAVIN, HJ;WIERASZKO, A;SEYFRIED, TN
在成年正常C57 BL/6 J（B6）和El（癫痫）小鼠的海马切片中研究了推定神经递质[天冬氨酸、谷氨酸和γ-氨基丁酸（GABA）]的释放。 El小鼠是颞叶癫痫的遗传模型，平均有86次癫痫发作。 将B6和E1海马切片组（400 μ m厚）在存在或不存在钙的情况下在一系列正常和高钾（60 mM）缓冲液中孵育。 在每个小鼠品系中比较了钙依赖性和钙非依赖性钾诱导的氨基酸释放。 内源性氨基酸的释放使用具有电化学检测的液相色谱法测量，并表示为每毫升孵育缓冲液每分钟孵育每切片释放的氨基酸的皮摩尔数+/- SEM。 在钙依赖性钾诱发的谷氨酸释放（18.20 +/- 2.62和15.41 +/- 3.56）或GABA（17.28 +/- 2.90和12.73 +/- 1.37）方面，El和B6小鼠之间没有发现显著差异。 然而，E1小鼠（6.62 +/- 0.69）中的天冬氨酸释放显著高于B6小鼠（3.31 +/- 0.72）。 这些发现表明，增强天冬氨酸释放可能与癫痫发作的表达在El小鼠。
The release of putative neurotransmitters [aspartate, glutamate, and gamma-aminobutyric acid (GABA)] was studied in hippocampal slices from adult normal C57BL/6J (B6) and El (epileptic) mice. The El mice, a genetic model of temporal lobe epilepsy, had an average of 86 seizures. Sets of B6 and El hippocampal slices (400 mu-m thick) were incubated in a series of normal and high potassium (60 mM) buffers in the presence or absence of calcium. The calcium-dependent and calcium-independent potassium-induced release of amino acids was compared in each mouse strain. Release of endogenous amino acids was measured using liquid chromatography with electrochemical detection and was expressed as picomoles of amino acid released per milliliter of incubation buffer per minute of incubation per slice +/- SEM. No significant differences were found between the El and B6 mice for the calcium-dependent potassium-evoked release of glutamate (18.20 +/- 2.62 and 15.41 +/- 3.56), or GABA (17.28 +/- 2.90 and 12.73 +/- 1.37), respectively. Aspartate release, however, was significantly higher in the El mice (6.62 +/- 0.69) than in the B6 mice (3.31 +/- 0.72). These findings suggest that enhanced aspartate release may be related to seizure expression in El mice.