Identification of amplified genes in a patient with acute myeloid leukemia and double minute chromosomes
Identification of amplified genes in a patient with acute myeloid leukemia and double minute chromosomes
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DOI：
10.1016/s0165-4608(99)00018-7
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发表时间：
1999-09-01
影响因子：
--
通讯作者：
Morris, CM
中科院分区：
文献类型：
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作者：
Crossen, PE;Morrison, MJ;Morris, CM
对1例具有双微小染色体和复杂核型异常的急性髓系白血病（M2）进行了细胞遗传学和分子遗传学分析。比较基因组杂交（CGH）显示，8 q24区域，包含MYC癌基因没有扩增。相反，鉴定了染色体区域11 q23--> qter和9 p11--> pter的扩增。Southern杂交分析证实了CGH结果，并显示位于11 q23-> 24的ETS 1、FLI 1、SRPR、NFRKB和KCNJ 5基因扩增，而位于11 q23的MLL没有扩增。此外，IFN β 1和CDKN 2A基因在9 p扩增，但程度较低。这是第一例双微小染色体急性髓细胞白血病病例，不涉及MYC或MLL基因扩增。(C)Elsevier Science Inc.，1999. All rights reserved.
A case of acute myeloid leukemia (M2) with double minute chromosomes and complex karyotypic abnormalities was analyzed cytogenetically and molecularly. Comparative genomic hybridization (CGH) showed that the 8q24 region that contains the MYC oncogene was not amplified. Instead, amplification of chromosomal regions 11q23 --> qter and 9p11 --> pter was identified. Southern blot analysis confirmed the CGH findings and showed that the ETS1, FLI1, SRPR, NFRKB, and KCNJ5 genes located at 11q23 --> 24 were amplified, whereas the MLL at 11q23 was not amplified. Additionally, the IFN beta 1 and CDKN2A genes at 9p were amplified, but to a lesser degree. This is the first example of a case of acute myeloid leukemia with double minute chromosomes that has not involved amplification of either the MYC or the MLL genes. (C) Elsevier Science Inc., 1999. All rights reserved.