Influence of corticosterone on growth, home-cage activity, wheel running, and aerobic capacity in house mice selectively bred for high voluntary wheel-running behavior
Influence of corticosterone on growth, home-cage activity, wheel running, and aerobic capacity in house mice selectively bred for high voluntary wheel-running behavior
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DOI：
10.1016/j.physbeh.2018.10.001
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发表时间：
2019-01-01
影响因子：
2.9
通讯作者：
Garland, Theodore, Jr.
中科院分区：
文献类型：
--
作者：
Singleton, Jennifer M.;Garland, Theodore, Jr.
糖皮质激素是一类代谢激素，影响广泛的性状（例如，行为、骨骼生长、肌肉维持、葡萄糖代谢）和循环糖皮质激素（如皮质酮）浓度的变化，在自然个体变化水平上，与内分泌失调有关，或来自外源性补充，具有多种影响。循环皮质酮浓度的变化也会影响运动行为的多个方面，包括运动的动机和身体能力。为了进一步研究皮质酮在运动行为和相关性状中的作用，我们利用了一项长期实验中的实验室小鼠，该实验室小鼠选择性地进行高水平的自愿运动。与四个未选择的对照（C）系相比，来自四个重复的高跑步者（HR）系的小鼠具有相似的高至2倍的基线循环皮质酮浓度以及相似的高至3倍的每天自主轮跑、当被剥夺轮时更高的家笼活动、更高的最大有氧能力和更小的体型;所有这些差异都可能受到循环皮质酮的调节。从断奶到8周龄，我们在HR和C雄性小鼠的饮用水中给予50 μ g/mL皮质酮-21-半琥珀酸酯。与来自C系的小鼠相比，HR小鼠具有更高的内源性皮质酮水平;更高的每日轮跑距离、持续时间和速度;更高的强迫运动期间最大耗氧量（VO（2）max）;在高架十字迷宫的封闭臂中花费更多时间;并且具有更大的生殖脂肪垫。对于HR和C小鼠，皮质酮治疗强烈抑制内源性循环皮质酮水平，降低生长速度和成年体重，增加食物和水的消耗（均根据体重调整），增加高架十字迷宫闭合臂的进入次数，减少笼内活动（总强度和平均强度），减少轮跑距离和最大速度，并降低VO（2）max。在亚机体水平，皮质酮治疗减少相对肾上腺，肝脏，小腿三头肌肌肉质量，以及尾长，但增加皮下和生殖脂肪垫质量，以及红细胞压积。总的来说，从健康的角度来看，HR和C小鼠对皮质酮补充剂的反应都是“负面的”。这些结果对理解基线皮质酮水平的演变和压力相关的活动水平的影响具有重要意义。他们还建议，经历长时间糖皮质激素治疗的患者可能会受益于尝试增加他们的体力活动作为一种辅助。
Glucocorticoids, a class of metabolic hormones, impact a wide range of traits (e.g., behavior, skeletal growth, muscle maintenance, glucose metabolism), and variation in concentrations of circulating glucocorticoids (such as corticosterone), at the level of natural individual variation, in relation to endocrine disorders, or from exogenous supplementation, have manifold effects. Changes in circulating corticosterone concentrations can also impact multiple aspects of locomotor behavior, including both motivation and physical ability for exercise. To examine further the role of corticosterone in locomotor behavior and associated traits, we utilized laboratory house mice from a long-term experiment that selectively breeds for high levels of voluntary exercise. As compared with four non-selected control (C) lines, mice from the four replicate High Runner (HR) lines have similar to 2-fold higher baseline circulating corticosterone concentrations as well as similar to 3-fold higher voluntary wheel running on a daily basis, higher home-cage activity when deprived of wheels, higher maximal aerobic capacity, and smaller body size; potentially, all of these differences could be modulated by circulating corticosterone. We administered 50 mu g/mL corticosterone-21-hemisuccinate in the drinking water of both HR and C male mice from weaning through similar to 8 weeks of age. As compared with mice from C lines, HR mice had higher endogenous corticosterone levels; higher daily wheel-running distance, duration, and speed; higher maximal oxygen consumption during forced exercise (VO(2)max); spent more time in the closed arms of an elevated plus maze; and had larger reproductive fat pads. For both HR and C mice, corticosterone treatment strongly suppressed endogenous circulating corticosterone levels, decreased growth rate and adult body mass, increased food and water consumption (both adjusted for body mass), increased entries into closed arms of an elevated plus maze, decreased home-cage activity (total and average intensity), decreased wheel-running distance and maximum speed, and decreased VO(2)max. At the suborganismal level, corticosterone treatment decreased relative adrenal, liver, and triceps surae muscle mass, as well as tail length, but increased both subdermal and reproductive fat pad masses, as well as hematocrit. Overall, the responses of both HR and C mice to corticosterone supplementation were "negative" from a health perspective. These results have significant implications for understanding both the evolution of baseline corticosterone levels and stress-related effects on activity levels. They also suggest that patients experiencing extended periods of glucocorticoid treatment might benefit from attempts to increase their physical activity as an adjuvant.