Analytical strategies for LC-MS-based targeted metabolomics
Analytical strategies for LC-MS-based targeted metabolomics
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DOI：
10.1016/j.jchromb.2008.04.031
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发表时间：
2008-08-15
影响因子：
3
通讯作者：
Rabinowitz, Joshua D.
中科院分区：
文献类型：
--
作者：
Lu, Wenyun;Bennett, Bryson D.;Rabinowitz, Joshua D.
质谱法的最新进展使得能够改进内源性代谢物的分析。在这里，我们讨论了与开发用于靶向代谢组学的液相色谱-电喷雾电离-质谱方法相关的几个问题（即，定量分析几十到几百种特定的代谢物）。样品制备和液相色谱方法进行了讨论，眼睛对处理的挑战与多样性的代谢物类平行。有证据表明，加热的电喷雾电离（ESI）通常提供了改善的信号相比，更传统的未加热的ESI。适用于以多反应监测（MRM）模式操作的三重四极杆质谱和高质量分辨率全扫描质谱的靶向代谢组学（例如，飞行时间，Orbitrap）。我们认为，这两种方法都是可行的解决方案，当目标分析物的数量更有限时，MRM是首选。而全扫描由于其桥接靶向和非靶向代谢组学的潜在能力而被优选。(C)2008 Elsevier B. V.保留所有权利。
Recent advances in mass spectrometry are enabling improved analysis of endogenous metabolites. Here we discuss several issues relevant to developing liquid chromatography-electrospray ionization-mass spectrometry methods for targeted metabolomics (i.e., quantitative analysis of dozens to hundreds of specific metabolites). Sample preparation and liquid chromatography approaches are discussed, with an eye towards the challenge of dealing with a diversity of metabolite classes in parallel. Evidence is presented that heated electrospray ionization (ESI) generally gives improved signal compared to the more traditional unheated ESI. Applicability to targeted metabolomics of triple quadrupole mass spectrometry operating in multiple reaction monitoring (MRM) mode and high mass resolution full scan mass spectrometry (e.g., time-of-flight, Orbitrap) are described. We suggest that both are viable solutions, with MRM preferred when targeting a more limited number of analytes. and full scan preferred for its potential ability to bridge targeted and untargered metabolomics. (C) 2008 Elsevier B.V. All rights reserved.