Mixed hemimicelles solid-phase extraction of cephalosporins in biological samples with ionic liquid-coated magnetic graphene oxide nanoparticles coupled with high-performance liquid chromatographic analysis.
Mixed hemimicelles solid-phase extraction of cephalosporins in biological samples with ionic liquid-coated magnetic graphene oxide nanoparticles coupled with high-performance liquid chromatographic analysis.
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DOI：
10.1016/j.chroma.2016.05.071
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发表时间：
2016-07
期刊：
影响因子：
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通讯作者：
Jianrong Wu;Hongyan Zhao;Deli Xiao;P. Chuong;Jia He;Hua He
中科院分区：
文献类型：
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作者：
Jianrong Wu;Hongyan Zhao;Deli Xiao;P. Chuong;Jia He;Hua He
建立了一种基于磁性氧化石墨烯（Fe 3 O 4/GO）和离子液体（IL）的混合半胶束固相萃取法，用于同时萃取和测定人尿中痕量头孢菌素类抗生素。利用1-十六烷基-3-甲基溴化咪唑（C16 mimBr）修饰后的氧化石墨烯的高比表面积和优异的吸附能力，将其应用于固相萃取（SPE）过程。综合考察了磁性氧化石墨烯的zeta电位、磁性氧化石墨烯用量、表面活性剂用量、溶液pH值、离子强度、萃取时间、解吸条件等因素对萃取回收率的影响。提出了使用不同表面活性剂涂覆的Fe 3 O 4/GO NPs作为吸附剂的比较研究。所有校准曲线均具有良好的线性（R2> 0.9987）。头孢菌素类的LOD范围为0.6 - 1.9 ng mL− 1，LOQ分别为1.5 - 5.5。生物样品的回收率为84.3%~ 101.7%，相对标准偏差为1.7%~ 6.3%。基于离子液体和Fe_3O_4/GO纳米粒子磁性分离的混合半胶束磁性固相萃取（MSPE）方法已成功地应用于复杂生物样品的前处理。
A novel mixed hemimicelles solid phase extraction based on magnetic graphene oxide (Fe3O4/GO) and ionic liquid (IL) was developed for the simultaneous extraction and determination of trace cephalosporins in spiked human urine. The high surface area and excellent adsorption capacity of the graphene oxide after modification with1-hexadecyl-3-methylmidazoliumbromide(C16mimBr) were utilized adequately in the solid phase extraction(SPE) process. A comprehensive study of the parameters affecting the extraction recovery, such as the zeta-potential of magnetic graphene oxide, amounts of magnetic graphene oxide and surfactant, pH of solution, ionic strength, extraction time, and desorption condition were optimized. A comparative study on the use of different surfacant-coated Fe3O4/GO NPs as sorbents was presented. Good linearity (R2> 0.9987) for all calibration curves was obtained. The LODs were ranged between 0.6 and 1.9 ng mL−1for the cephalosporins and the LOQs were 1.5 to 5.5, respectively. Satisfactory recoveries(84.3% to 101.7%)and low relative standard deviations from 1.7% to 6.3% in biological matrices were achieved. The mixed hemimicelles magnetic SPE (MSPE) method based on ILs and Fe3O4/GO NPs magnetic separation has ever been successfully used for pretreatment of complex biological samples.