BMP-4 regulates the dorsal-ventral differences in FGF/MAPKK-mediated mesoderm induction in Xenopus.
BMP-4 regulates the dorsal-ventral differences in FGF/MAPKK-mediated mesoderm induction in Xenopus.
复制标题
BMP-4 调节非洲爪蟾 FGF/MAPKK 介导的中胚层诱导中的背腹差异。
DOI：
10.1006/dbio.1995.0019
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发表时间：
1995
影响因子：
2.7
通讯作者：
Kimelman,D
中科院分区：
文献类型：
--
作者：
Northrop,J;Woods,A;Seger,R;Suzuki,A;Ueno,N;Krebs,E;Kimelman,D
最近对Xenopus发育的研究揭示了一系列越来越复杂的诱导、预模式化和能力信号，这些信号是正确的中胚层形成所必需的。在这项研究中，我们建立了成纤维细胞生长因子（FGF）信号通过丝裂原活化蛋白激酶激酶（MAPKK）诱导中胚层基因表达。我们证明MAPKK的部分激活形式恢复了中胚层基因Xcad-3和Xbra的表达，这些基因被显性负性FGF受体（ΔFGFR）消除。与之前报道的ΔFGFR结果相似，MAPKK的显性阴性表达（MAPKKD）优先消除Xcad-3和Xbra的背侧表达。我们测试了骨形态发生蛋白-4（BMP-4）的区域定位是否可以解释为什么MAPKKD和ΔFGFR都消除了Xcad-3和Xbra的背侧而不是腹侧表达。我们表明，BMP-4的异位表达可以消除Xcad-3和Xbra的背侧表达。4足以维持含有ΔFGFR的Xcad-3和Xbrain胚胎的背侧表达，并且显性负性BMP受体的表达减少了ΔFGFR胚胎的背腹差异。这些结果表明，BMP-4的区域定位负责FGF/MAPKK介导的中胚层诱导的背腹不对称。
Recent studies onXenopusdevelopment have revealed an increasingly complex array of inductive, prepatterning, and competence signals that are necessary for proper mesoderm formation. In this study, we establish that fibroblast growth factor (FGF) signals through mitogen-activated protein kinase kinase (MAPKK) to induce mesodermal gene expression. We demonstrate that a partially activated form of MAPKK restores expression of the mesodermal genesXcad-3andXbra,eliminated by the dominant-negative FGF receptor (ΔFGFR). Similar to the results reported earlier with ΔFGFR, expression of a dominant-negative form of MAPKK (MAPKKD) preferentially eliminates the dorsal expression ofXcad-3andXbra.We tested whether the regional localization of bone morphogenetic protein-4 (BMP-4) could explain why both MAPKKD and ΔFGFR eliminate the dorsal and not the ventral expression ofXcad-3andXbra.We show that ectopic expression of BMP-4 is sufficient to maintain the dorsal expression ofXcad-3andXbrain embryos containing ΔFGFR and that expression of a dominant-negative BMP receptor reduces the dorsal–ventral differences in ΔFGFR embryos. These results indicate that regional localization of BMP-4 is responsible for the dorsal–ventral asymmetry in FGF/MAPKK-mediated mesoderm induction.