Immunohistochemical localization of components of the insulin-like growth factor system in human permanent teeth
Immunohistochemical localization of components of the insulin-like growth factor system in human permanent teeth
复制标题
DOI：
10.1016/j.archoralbio.2005.10.005
复制
发表时间：
2006-05-01
影响因子：
3
通讯作者：
Jaeger, Andreas
中科院分区：
文献类型：
--
作者：
Goetz, Werner;Heinen, Michael;Jaeger, Andreas
越来越多的证据表明，胰岛素样生长因子（IGF）系统在口腔牙齿面部组织和器官的生物学中发挥着重要作用，包括牙周组织的发育、稳态和再生。为了获得人类恒牙中 IGF 成分的出现和分布的基本数据，我们使用针对配体 IGF-I 和 -II、IGF1 受体 (IGF1R) 等的单克隆和多克隆抗体，通过免疫组织化学方法研究了 25 颗拔出、脱钙和石蜡包埋的牙齿。六种 IGF 结合蛋白（IGFBP-1 至 -6）。在粘附牙周的细胞外基质（ECM）中。观察韧带（PDL）、IGF-I、-II以及IGFBP-1和-6的免疫反应性。 PDL成纤维细胞显示IGF1 R的免疫染色。对于牙骨质，在无细胞牙骨质中仅可以检测到IGF-II，而插入Sharpey纤维的外牙骨质层与除IGFBP-4和-6之外的所有抗体发生反应。在牙髓中，主要纤维化区域和小齿周围区域对 IGF-I、IGFBP-1、-3、-5 和 -6 具有免疫反应性。对前牙本质和成牙本质细胞过程进行 IGF-I 和 IGFBP-3 染色。人类恒牙中 IGF 系统成分的空间定向出现表明 IGF 的特定功能可能位于特定的组织区室中。在牙骨质中，发现了几种 IGF 成分，表明其在组织稳态或附着中的作用。 PDL 可能充当可能与 ECM 成分结合的 IGF 的储存库。 PDL 成纤维细胞随后可以以旁分泌方式做出反应。在牙髓中，IGF 系统可能参与成牙本质细胞生物学、纤维化和小齿形成。 (c) 2005 Elsevier Ltd. 保留所有权利。
There is growing evidence that the insulin-like growth factor (IGF) system plays an important rote in the biology of oro-dento-facial tissues and organs, including the development, homeostasis and regeneration of the periodontium. To obtain basic data on the occurrence and distribution of IGF components in human permanent teeth we immunohistochemically investigated 25 extracted, decalcified and paraffin-embedded teeth using mono and polyclonal antibodies against the ligands IGF-I and -II, the IGF1 receptor (IGF1R) and all. six IGF binding proteins (IGFBP-1 to -6). In the extracellular matrix (ECM) of the adhering periodontal. Ligament (PDL), immunoreactivity for IGF-I, -II and IGFBP-1 and -6 was observed. PDL fibroblasts showed immunostaining for the IGF1 R. For the cementum, in the acellular cementum only IGF-II could be detected, while outer cementum layers with inserting Sharpey's fibers reacted with all antibodies applied except for IGFBP-4 and -6. In the pulp, mainly fibrotic areas and areas around denticles were immunoreactive for IGF-I, IGFBP-1, -3, -5 and -6. Predentin and odontoblastic processes were stained for IGF-I and IGFBP-3. The spatially oriented occurrence of components of the IGF system in human permanent teeth indicates that specific functions of the IGFs may be Localized in particular tissue compartments. In the cementum, several IGF components were found indicating roles in tissue homeostasis or attachment. The PDL may function as a reservoir for IGFs probably bound to ECM components. PDL fibroblasts could then respond in a paracrine manner. In the pulp, the IGF system may be involved in odontoblast biology, fibrosis and denticle formation. (c) 2005 Elsevier Ltd. All rights reserved.