Integrated study of circRNA, lncRNA, miRNA, and mRNA networks in mediating the effects of testicular heat exposure
Integrated study of circRNA, lncRNA, miRNA, and mRNA networks in mediating the effects of testicular heat exposure
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circRNA、lncRNA、miRNA 和 mRNA 网络在介导睾丸热暴露影响中的综合研究
DOI：
10.1007/s00441-021-03474-z
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发表时间：
2021-05-20
影响因子：
3.6
通讯作者：
Liang, Meng
中科院分区：
文献类型：
--
作者：
Hu, Ke;He, Chaofan;Liang, Meng
世界卫生组织已经认识到，睾丸功能与温度有关。由职业因素、生活方式和临床疾病引起的睾丸热暴露可导致不同程度的生殖问题。这项研究的目的是揭示转录调控网络及其在调节睾丸热暴露的影响中的潜在关键作用。睾丸组织收集自一组接受阴囊热暴露的小鼠和一组对照组。从两组中提取RNA并用于高通量测序。应用差异转录组分析，与对照组相比，热暴露后小鼠睾丸组织中有172个CircRNAs、279个miRNAs、465个lncRNAs和2721个mRNAs显著差异表达。通过基因本体论(GO)术语和京都基因和基因组百科全书(KEGG)的途径分析，差异表达的LncRNA和mRNAs被发现在减数分裂细胞周期(GO：0051321)、细胞质(GO：0005737)、膜筏(GO：0045121)、丝裂原活化蛋白K信号转导(Mmu04010)、嘌呤代谢(Mmu00230)和同源重组(Mmu03440)中具有潜在的重要功能。通过竞争的内源RNA调控网络分析，预测一些最上调和下调的lncRNAs和CircRNAs与大量的miRNAs和mRNAs相关，并得到了分子生物学实验的验证。本研究提供了睾丸热暴露模型的高通量测序数据，为进一步研究与睾丸温度升高相关的男性生殖疾病相关的CircRNAs、miRNAs和lncRNAs奠定了基础。
The World Health Organization has recognized that testicular function is temperature dependent. Testicular heat exposure caused by occupational factors, lifestyle, and clinical diseases can lead to different degrees of reproductive problems. The aim of this study was to reveal the transcriptional regulatory network and its potential crucial roles in mediating the effects of testicular heat exposure. Testicular tissue was collected from a group of mice subjected to scrotal heat exposure as well as a control group. RNA was isolated from both groups and used for high-throughput sequencing. Using differential transcriptome expression analysis, 172 circRNAs, 279 miRNAs, 465 lncRNAs, and 2721 mRNAs were identified as significantly differentially expressed in mouse testicular tissue after heat exposure compared with the control group. Through Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, differentially expressed lncRNAs and mRNAs were found to have potentially important functions in meiotic cell cycle (GO:0051321), cytoplasm (GO:0005737), membrane raft (GO:0045121), MAPK signaling (mmu04010), purine metabolism (mmu00230), and homologous recombination (mmu03440). Some of the most upregulated and downregulated lncRNAs and circRNAs were predicted to be associated with numerous miRNAs and mRNAs through competing endogenous RNA regulatory network analysis, which were validated with molecular biology experiments. This research provides high-throughput sequencing data of a testicular heat exposure model and lays the foundation for further study on circRNAs, miRNAs, and lncRNAs that are involved in male reproductive diseases related to elevated testicular temperature.