Soil bacterial and fungal community dynamics in relation to Panax notoginseng death rate in a continuous cropping system
Soil bacterial and fungal community dynamics in relation to Panax notoginseng death rate in a continuous cropping system
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连作制度下土壤细菌和真菌群落动态与三七死亡率的关系
DOI：
10.1038/srep31802
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发表时间：
2016-08-23
影响因子：
4.6
通讯作者：
Chen, Shilin
中科院分区：
文献类型：
--
作者：
Dong, Linlin;Xu, Jiang;Chen, Shilin
三七是一种珍贵的中药材，在连作条件下死亡率高。土壤微生物群落的变化被认为是三七死亡的主要原因，尽管导致作物歉收的类群仍然没有确定。本研究采用高通量测序方法来表征微生物群落的变化，并筛选与死亡率相关的微生物类群。三七种植3年后，土壤真菌多样性显著下降。死亡率与真菌多样性呈显著负相关，表明真菌多样性可能是土壤健康的一个潜在的生物指示剂。正相关分析表明，伯克霍尔德菌目、共生营养杆菌科、桃蚜壳属、褐球腔菌属、镰刀菌属和茎点霉属对病株定殖的适应性较强。尖孢镰刀菌（R = 0.841，P0.05）和罗氏褐球腔菌（R = 0.830，P0.05）的相对丰度与死亡率呈正相关。F.尖孢菌是三七根腐病的病原菌，可引起三七幼苗死亡。相关系数分析表明，热芽孢菌科、放线菌科、水门汀科、卷枝孢菌科和锥孢菌属可能是病原菌的拮抗菌，且穿孔锥孢菌的相对丰度与死亡率呈负相关。我们的研究结果提供了一个动态的微生物群落的概述，并提出了一个明确的范围，筛选有益的微生物和病原体的三七。
Notoginseng (Panax notoginseng), a valuable herbal medicine, has high death rates in continuous cropping systems. Variation in the soil microbial community is considered the primary cause of notoginseng mortality, although the taxa responsible for crop failure remains unidentified. This study used high-throughput sequencing methods to characterize changes in the microbial community and screen microbial taxa related to the death rate. Fungal diversity significantly decreased in soils cropped with notoginseng for three years. The death rate and the fungal diversity were significantly negatively correlated, suggesting that fungal diversity might be a potential bioindicator of soil health. Positive correlation coefficients revealed that Burkholderiales, Syntrophobacteraceae, Myrmecridium, Phaeosphaeria, Fusarium and Phoma were better adapted to colonization of diseased plants. The relative abundance of Fusarium oxysporum (R = 0.841, P 0.05) and Phaeosphaeria rousseliana (R = 0.830, P 0.05) were positively associated with the death rate. F. oxysporum was a pathogen of notoginseng root-rot that caused seedling death. Negative correlation coefficients indicated that Thermogemmatisporaceae, Actinosynnemataceae, Hydnodontaceae, Herpotrichiellaceae and Coniosporium might be antagonists of pathogens and the relative abundance of Coniosporium perforans was negatively correlated with the death rate. Our findings provide a dynamic overview of the microbial community and present a clear scope for screening beneficial microbes and pathogens of notoginseng.