Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes.
Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes.
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DOI：
10.5625/lar.2018.34.4.147
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发表时间：
2018-12-01
影响因子：
2.9
通讯作者：
Fang, Sungsoon
中科院分区：
文献类型：
--
作者：
Kim, Hyeonhui;Kim, Minki;Fang, Sungsoon
由于小鼠和人类在遗传和病理生理学上的相似性，基因工程小鼠模型通常是研究人类疾病的首选模型。特别是，Cre-loxP系统被广泛用作生成条件式的一个完整的实验工具。该系统使研究人员能够以组织/细胞(空间控制)和/或时间(时间控制)特定的方式研究感兴趣的基因。到目前为止，已经产生了各种组织特异性Cre驱动鼠系，新的Cre系仍在开发中。本文简要介绍了Cre-loxP系统和几种常用的组织特异性Cre重组酶表达启动子。此外，我们最后介绍了一些可用的网站链接，这些网站提供了有关Cre鼠标线的详细信息，包括它们的特征。
Genetically engineered mouse models are commonly preferred for studying the human disease due to genetic and pathophysiological similarities between mice and humans. In particular, Cre-loxP system is widely used as an integral experimental tool for generating the conditional. This system has enabled researchers to investigate genes of interest in a tissue/cell (spatial control) and/or time (temporal control) specific manner. A various tissue-specific Cre-driver mouse lines have been generated to date, and new Cre lines are still being developed. This review provides a brief overview of Cre-loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase. Also, we finally introduce some available links to the Web sites that provides detailed information about Cre mouse lines including their characterization.