Microbiome of the placenta in pre-eclampsia supports the role of bacteria in the multifactorial cause of pre-eclampsia
Microbiome of the placenta in pre-eclampsia supports the role of bacteria in the multifactorial cause of pre-eclampsia
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DOI：
10.1111/jog.12619
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发表时间：
2015-05-01
影响因子：
1.6
通讯作者：
Dissanayake, Vajira H. W.
中科院分区：
文献类型：
--
作者：
Amarasekara, Ranmalee;Jayasekara, Rohan W.;Dissanayake, Vajira H. W.
目的本研究的目的是检测，鉴定，定量和比较的细菌存在于胎盘组织中的妇女与先兆子痫与normo-pressurepregnanceptivewomen.Material和MethodsPlacentaltissue标本收集从55个先兆子痫的产妇（病例）和55个匹配的正常血压的孕妇（对照组）在分娩时剖宫产。基因分型分两个阶段进行。首先，通过16 S rRNA基因聚合酶链反应（PCR）筛选样本中是否存在细菌。接下来，PCR阳性的16 S rRNA基因的样本进行了筛选，通过下一代测序的Illumina MiSeq platform.ResultsSeven（12.7%）的胎盘组织样本的妇女先兆子痫PCR阳性。所有对照组胎盘样本均为阴性（P=0.006）。通过下一代测序揭示了七个样本的完整微生物组。存在的微生物包括蜡样芽孢杆菌、李斯特菌、沙门氏菌、埃希氏菌（所有这些通常都与胃肠道感染有关）;肺炎克雷伯菌和缺氧杆菌（两者通常都与呼吸道感染有关）;和Variovorax，Prevotella，Porphyromonas，以及小杆菌属（所有这些通常都与牙周炎有关）结论本研究证实了细菌在先兆子痫患者胎盘组织中的存在，并支持细菌在先兆子痫多因素病因中的作用。
AimThis study was aimed at detecting, identifying, quantifying and comparing the bacteria present in the placental tissues of women with pre-eclampsia with that of normotensive pregnant women.Material and MethodsPlacental tissue samples were collected from 55 primiparous women with pre-eclampsia (cases) and 55 matched primiparous normotensive pregnant women (controls) at the time of delivery by cesarean section. Genotyping was carried out in two stages. First the samples were screened for the presence of bacteria by polymerase chain reaction (PCR) for the 16S rRNA gene. Next, the samples that were PCR-positive for the 16S rRNA gene were screened by next-generation sequencing on an Illumina MiSeq platform.ResultsSeven (12.7%) placental tissue samples from women with pre-eclampsia were PCR-positive. All the placental samples from control women were negative (P=0.006). The complete microbiome of the seven samples was revealed through next-generation sequencing. The organisms that were present included Bacillus cereus, Listeria, Salmonella, Escherichia (all of which are usually associated with gastrointestinal infection); Klebsiella pneumonia and Anoxybacillus (both of which are usually associated with respiratory tract infections); and Variovorax, Prevotella, Porphyromonas, and Dialister (all of which are usually associated with periodontitis).ConclusionsThis study confirms the presence of bacteria in the placental tissues of a subset of women with pre-eclampsia and supports the role of bacteria in the multifactorial cause of pre-eclampsia.