3D-printed bioactive and biodegradable hydrogel scaffolds of alginate/gelatin/cellulose nanocrystals for tissue engineering
3D-printed bioactive and biodegradable hydrogel scaffolds of alginate/gelatin/cellulose nanocrystals for tissue engineering
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DOI：
10.1016/j.ijbiomac.2020.12.011
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发表时间：
2021-01-15
影响因子：
8.2
通讯作者：
Lim, Ki-Taek
中科院分区：
文献类型：
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作者：
Dutta, Sayan Deb;Hexiu, Jin;Lim, Ki-Taek
由海藻酸盐、明胶和纤维素纳米晶体 (CNC) 组成的 3D 打印混合可生物降解水凝胶的制备可为骨组织工程 (BTE) 应用中的细胞增殖、粘附、营养物质交换和基质矿化提供有利的环境。与纯聚合物支架相比，混合支架表现出增强的机械强度。分别通过DAPI染色、活死实验、茜素红-S（ARS）染色、实时PCR（qRT-PCR）和μ CT分析评估打印支架的生物相容性、分化潜力和骨再生潜力。与对照相比，1% CNC/Alg/Gel 支架的细胞增殖增强。细胞充分粘附在支架上并呈现扁平结构。与对照相比，在 1% CNC/Alg/Gel 支架的存在下观察到矿化有所改善，显示了它们的矿化效率。与对照相比，1% CNC/Alg/Gel 的成骨特异性基因标记物（Runx2、ALP、BMP-2、OCN、OPN、BSP 和 COL1）的表达显着增强，表明其成骨潜力。此外，在颅骨临界尺寸缺损（CCD-1）模型中，支架治疗组的骨形成比对照组有所增强，这表明它们的骨再生潜力有所改善。因此，所制造的支架具有作为组织工程生物材料进行探索的潜力。 (C) 2020 Elsevier B.V. 保留所有权利。
The 3D-printed hybrid biodegradable hydrogels composed of alginate, gelatin, and cellulose nanocrystals (CNCs) were prepared to provide a favorable environment for cell proliferation, adhesion, nutrients exchange, and matrix mineralization for bone tissue engineering (BTE) applications. The hybrid scaffolds exhibited enhanced mechanical strength compared to the pure polymer scaffolds. The biocompatibility, differentiation potential, and bone regeneration potential of the printed scaffolds were evaluated by DAPI staining, live-dead assay, alizarin Red-S (ARS) staining, real-time PCR (qRT-PCR), and mu CT analysis, respectively. Enhanced cell proliferation has occurred 1% CNC/Alg/Gel scaffolds compared to the control. The cells were adequately adhered to the scaffold and exhibited the flattened structure. Improved mineralization was observed in the 1% CNC/Alg/Gel scaffolds' presence than the control, showing their mineralization efficiency. A significant enhancement in the expression of osteogenic-specific gene markers (Runx2, ALP, BMP-2, OCN, OPN, BSP, and COL1) has occurred with 1% CNC/Alg/Gel than the control, indicating their osteogenic potential. Furthermore, enhanced bone formation was observed in the scaffolds treated groups than the control in the calvaria critical-sized defects (CCD-1) model, suggesting their improved bone regeneration potential. Therefore, the fabricated scaffolds have the potential to explore as a biomaterial for tissue engineering. (C) 2020 Elsevier B.V. All rights reserved.