Quantitative label-free screening for antibodies using scattering biophotonic microarray imaging
Quantitative label-free screening for antibodies using scattering biophotonic microarray imaging
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DOI：
10.1016/j.ab.2009.08.008
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Shaw, Andrew M.
中科院分区：
文献类型：
--
作者：
Olkhov, Rouslan V.;Shaw, Andrew M.
基于抗原蛋白功能化的金纳米粒子的生物光子阵列已被用于测定溶液中各自抗体的浓度。四种蛋白-纤维蛋白原，牛血清白蛋白，转铁蛋白和c反应蛋白-被用来构建一个测试阵列，该实验重复多次。通过一系列校准实验确定抗体-抗原结合和解离速率常数。使用两个相关的动力学分析进行了未知抗体浓度的无标记测定。从这些结果来看，目前的阵列检测灵敏度为250 ng ml(-1)，使用8分钟动力学测量和16点平均检测的准确性为15%。(C) 2009爱思唯尔公司版权所有。
A biophotonic array based on gold nanoparticles functionalized with antigen proteins has been used to determine the concentrations of the respective antibodies in solution. Four proteins-fibrinogen, bovine serum albumin, transferrin, and C-reactive protein-were used to construct a test array with the assay repeated a number of times. The antibody-antigen association and dissociation rate constants were determined for the antibody assays from a series of calibration experiments. The label-free determination of the unknown antibody concentrations was performed using two related kinetic analyses. From these results, the current array assay sensitivity is 250 ng ml(-1) with an accuracy of 15% using an 8-min kinetic measurement and a 16-spot averaged assay. (C) 2009 Elsevier Inc. All rights reserved.