Antigenic Relationships among Five Reovirus-Like (RVL) Agents by Complement Fixation (CF) and Development of New Substitute CF Antigens for the Human RVL Agent of Infantile Gastroenteritis
Antigenic Relationships among Five Reovirus-Like (RVL) Agents by Complement Fixation (CF) and Development of New Substitute CF Antigens for the Human RVL Agent of Infantile Gastroenteritis
复制标题
通过补体固定 (CF) 确定五种呼肠孤病毒样 (RVL) 制剂之间的抗原关系以及开发婴儿胃肠炎人 RVL 制剂的新替代 CF 抗原
DOI：
10.3181/00379727-152-39434
复制
发表时间：
1976
影响因子：
--
通讯作者：
Annie L. Vaughn
中科院分区：
文献类型：
--
作者：
A. Kapikian;W. Cline;Hyun W. Kim;A. Kalica;R. Wyatt;D. Vankirk;R. Chanock;H. James;Annie L. Vaughn
在使用超免疫动物血清的相互补体结合（CF）试验中，发现人呼肠孤病毒样（HRVL）因子、内布拉斯加小牛腹泻病毒（NCDV）、幼鼠流行性腹泻（EDIM）病毒、猿猴因子（SA）-11和“O”（内脏）因子相似（如果不相同）。此外，在对35例排出HRVL因子的腹泻患者的配对血清进行的CF试验中，74%的患者出现了HRVL抗原感染的血清学证据，43%的患者出现了NCDV感染的血清学证据，51%的患者出现了EDIM病毒感染的血清学证据，57%的患者出现了SA-11感染的血清学证据，71%的患者出现了O因子感染的血清学证据。因此，除了先前已被描述为HRVL试剂的合适替代CF抗原的NCDV之外，SA-11、“0”和EDIM病毒也可用作HRVL试剂的替代抗原。然而，“0”试剂似乎是四种替代CF抗原中最有效的，因此当HRVL试剂不可用时应优先使用。“O”试剂与HRVL试剂一样有效，并且在检测血清应答方面比NCDV更有效。当同时用HRVL和“O”试剂检测血清时，检测HRVL试剂感染的效率最高，因为31例（89%）患者出现了一种或两种抗原感染的血清学证据。另外的替代CF抗原的HRVL剂的发现可能有意义的免疫预防人类疾病。感谢D博士。W.在统计分析和C先生的帮助下，E.班克斯，艾先生。威廉姆斯，B先生。安德鲁斯，杰克逊先生，D小姐。M. Bertran，S夫人J.凯利，罗伯特·P·查姆斯先生，L. P. Kendrick，C先生。S.奥斯本，B夫人。V. Armiger和夫人C。L.阿米格在这些研究中的援助。
Summary The human reovirus-like (HRVL) agent, Nebraska calf diarrhea virus (NCDV), epizootic diarrhea of infant mice (EDIM) virus, simian agent (SA)-11, and the “O” (offal) agent were found to be similar, if not identical, in reciprocal complement fixation (CF) tests employing hyperimmune animal sera. In addition, in CF tests with paired sera from 35 diarrhea patients who shed the HRVL agent, 74% developed serologic evidence of infection with the HRVL antigen, 43% with NCDV, 51% with EDIM virus, 57% with SA-11, and 71% with the “O” agent. Thus, in addition to the NCDV, which had previously been described as a suitable substitute CF antigen for the HRVL agent, the SA-11, “O,” and EDIM viruses may also be utilized as substitute antigens for the HRVL agent. However, the “O” agent appears to be the most efficient of the four substitute CF antigens and thus should be used preferentially when the HRVL agent is not available. The “O” agent was about as efficient as the HRVL agent and significantly more efficient than the NCDV for detecting seroresponses. The greatest efficiency for detecting infection with the HRVL agent resulted when sera were tested with both the HRVL and “O” agents as 31 (89%) of the patients developed serologic evidence of infection with one or both antigens. The finding of additional substitute CF antigens for the HRVL agent may have implications in the immuno-prophylaxis against human disease. We thank Dr. D. W. Alling for assistance in statistical analyses and Mr. C. E. Banks, Mr. E. Williams, Mr. B. Andrews, Mr. J. P. Jackson, Miss D. M. Bertran, Mrs. S. J. Kelly, Mr. Robert P. Chames, Mrs. L. P. Kendrick, Mr. C. S. Osborne, Mrs. B. V. Armiger, and Mrs. C. L. Armiger for assistance in these studies.