Studies of bystander effects in human fibroblasts using a charged particle microbeam
Studies of bystander effects in human fibroblasts using a charged particle microbeam
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DOI：
10.1080/095530098141087
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发表时间：
1998-12-01
影响因子：
2.6
通讯作者：
Michael, BD
中科院分区：
文献类型：
--
作者：
Prise, KM;Belyakov, OV;Michael, BD
目的：利用一种能靶向单个细胞的带电粒子微摄像头，研究细胞间效应在不同辐射反应中的作用。材料和方法：用X射线或α粒子常规照射原代人成纤维细胞，并在照射后不同时间对细胞微核或凋亡细胞的形成进行评分。结果：常规照射后3d，无论是X射线还是α粒子，成纤维细胞均表现出剂量依赖性微核和凋亡细胞的产生，低剂量α粒子的RBE值较高。使用微束将单个阿尔法粒子定向到一个群体中的四个细胞，仅在直接作用的基础上产生了比预期更多的微核和凋亡细胞。结论：这项研究为单独暴露于带电粒子的HIT细胞和非HIT细胞之间产生可传递的细胞间效应提供了直接证据。
Purpose: To determine the role of cell-to-cell mediated effects in the response of cells to different radiations by using a charged particle microbcam capable of targeting individual cells with counted particles.Materials and methods: Primary human fibroblasts were irradiated conventionally with X-rays or alpha-particles and scored for the induction of micronucleated or apoptotic cell formation at various times after irradiation. Cells were also individually irradiated with helium-3 particles from a microbeam and the distribution of damaged cells between hit and non-hit cells scored.Results: Conventionally exposed fibroblasts showed a dose-dependent production of micronucleated and apoptotic cells 3 days after irradiation for both X-rays and alpha-particles, with a high RBE value for alpha-particles at low doses. Targeting individual alpha-particles, using a microbeam, to four cells within a population produced more micronucleated and apoptotic cells than expected on the basis of a direct effect only.Conclusions: This study provides direct evidence for the production of transmissible, cell-to-cell effects between hit and non-hit cells individually exposed to charged particles.