Gene expression profiles of intracellular and membrane progesterone receptor isoforms in the mediobasal hypothalamus during pro-oestrus.
Gene expression profiles of intracellular and membrane progesterone receptor isoforms in the mediobasal hypothalamus during pro-oestrus.
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DOI：
10.1111/j.1365-2826.2009.01920.x
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发表时间：
2009-12
影响因子：
3.2
通讯作者：
Arbogast LA
中科院分区：
文献类型：
--
作者：
Liu B;Arbogast LA
黄体酮的作用是通过与特定受体的结合来实现的。孕酮受体(PR)的两种亚型(PrA和Prb)、三种膜孕酮受体亚型(MPRα、MPRβ和MPRγ)以及至少一种孕酮膜结合蛋白[PR膜组分1(Prmc1)]已在不同物种的生殖组织和脑中被发现。在本研究中，我们检测了发情前期大鼠下丘脑内侧基底核(MBH)中PR亚型、MPR亚型和PRmc1的基因表达模式。分别于发情第2天13.00h，动情前期09.00，13.00，17.00，22.00h，发情13.00h，用实时定量逆转录聚合酶链式反应(RT-PCR)定量检测各受体亚型的mRNA水平。对于PR，一对引物扩增PRA+PRB，第二对引物扩增PRB。不出所料，在MBH组织中，PRA+PRB的mRNA表达高于PRB。发情前期PRB基因表达全天升高，17.00h达到最高水平，发情前期13.00h和17.00h分别是发情第2天13.00h的2.4h和3.0h，差异有统计学意义，其中PRmc1表达最高，γ表达最低。发情前期13.00h和17.00h的MPRαβ含量是发情第2天13.00h的1.5倍，发情前期13.00h和17.00h的水平分别是发情第2天13.00h的2.5h和2.4h。PRA+PRB、MPRγ和PRMC1mRNA水平在发情前期差异不显著。这些结果表明，促发情午后胎盘出血中pRb、mprα和mprβ的高表达可能影响排卵前关键时期孕酮作用的基因组和非基因组机制。
Progesterone action is mediated by its binding to specific receptors. Two progesterone receptor (PR) isoforms (PRA and PRB), three membrane progesterone receptor (mPR) subtypes (mPRα, mPRβ and mPRγ) and at least one progesterone membrane-binding protein [PR membrane component 1 (PRmc1)] have been identified in reproductive tissues and brain of various species. In the present study, we examined gene expression patterns for PR isoforms, mPR subtypes and PRmc1 in the rat mediobasal hypothalamus (MBH) during pro-oestrus. The mRNA level for each receptor subtype was quantified by a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) at the time points: 13.00 h on dioestrous day 2; 09.00, 13.00, 17.00 and 22.00 h on pro-oestrus; and 13.00 h on oestrus. For PR, one primer set amplified PRA+PRB, whereas a second primer set amplified PRB. As expected, PRA+PRB mRNA expression was greater than PRB in MBH tissue. PRB mRNA levels increased throughout the day on pro-oestrus, with the highest levels being observed at 17.00 h. PRB mRNA levels in the MBH were increased by 2.4- and 3.0-fold at 13.00 and 17.00 h, respectively, on pro-oestrus compared to 13.00 h on dioestrous day 2. There were differential mRNA expression levels for mPRs and PRmc1 in the MBH, with the highest expression for PRmc1 and the lowest for mPRγ. The mPRα mRNA contents at 13.00 and 17.00 h on pro-oestrus were increased by 1.5-fold compared to that at 13.00 h on dioestrous day 2. The mPRβ mRNA levels at 13.00 and 17.00 h on pro-oestrus were 2.5- and 2.4-fold higher compared to that at 13.00 h on dioestrous day 2, respectively. PRA+PRB, mPRγ and PRmc1 mRNA levels did not vary on pro-oestrus. These findings suggest that the higher expression of PRB, mPRα and mPRβ in the MBH on pro-oestrous afternoon may influence both genomic and nongenomic mechanisms of progesterone action during the critical pre-ovulatory period.
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影响因子：
3.2
作者：
Leite, C. M.;Szawka, R. E.;Anselmo-Franci, J. A.
通讯作者：
Anselmo-Franci, J. A.
DOI：
10.1073/pnas.97.23.12816
发表时间：
2000-11-07
影响因子：
11.1
作者：
Krebs, CJ;Jarvis, ED;Pfaff, DW
通讯作者：
Pfaff, DW
影响因子：
11.4
作者：
KASTNER, P;KRUST, A;CHAMBON, P
通讯作者：
CHAMBON, P
影响因子：
4.1
作者：
Dufourny, L;Caraty, A;Skinner, DC
通讯作者：
Skinner, DC
影响因子：
4.8
作者：
Liu, Bin;Arbogast, Lydia A.
通讯作者：
Arbogast, Lydia A.